Srijayee Ghosh

Using Dark Dyes for Fast Super-Resolution Imaging: A Proof-of-Concept Study DNA-PAINT (Point Accumulation for Imaging in Nanoscale Topography) exploits the transient hybridization of a short fluorescent DNA imager strand to a complementary docking strand to achieve super-reso...

Can one use 'Dark' or non-emissive dyes for #fast #Super-Resolution #fluorescence 🌟 #microscopy?

In this work, we show the first proof-of-concept of Dark-RET (DRET) #DNA-PAINT, using a dark donor with a bright acceptor, for cellular imaging in <30 seconds. Link ⬇️

pubs.acs.org/doi/abs/10.1...

Done ✅: the www.nanodynamicslab.nl website is no more: one year after our move from the Netherlands to Switzerland (the 'Highlands'? 🤔), it was time to take the old website down.

You still find us here, at our @unibas.ch home: schmid.chemie.unibas.ch/en/ (revamp underway).

I am super happy to share our latest article entitled "Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy", now published in Science www.science.org/doi/10.1126/...
It was my postdoctoral work mit @sauer-lab.bsky.social Congrats to all the co-authors!

High-speed 10-plex DNA-PAINT with a larger sequence repertoire DNA-Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) enables multiplexed super-resolution imaging of biological samples. We expand the repertoire of speed-optimized DNA sequences fo...

Check out a preprint from our lab! We've uncovered all possible speed imager sequences for DNA-PAINT. You can now do 10-plex imaging in just a few hours with simple Exchange-PAINT. 🚀📸 #DNApaint #SuperResolution
www.biorxiv.org/content/10.1...

The known unknowns of the Hsp90 chaperone An exploration of the unanswered questions in how the molecular chaperone Hsp90 supports protein homeostasis, and how single-molecule techniques could drive future breakthroughs in answering them.

What a way to start 2025! Our review on the Known Unknowns of the Hsp90 Chaperone has been published!
Huge thanks to Laura Silbermann and Benjamin Vermeer for all of their efforts, and to @scisonja.bsky.social for the great collaboration :)
You can read it here:
elifesciences.org/articles/102...

A welcome move to boost Indian research.

Grateful for the opportunity to learn more about fluorogenic systems and the helpful discussions during the writing.

Fluorogenic binders would enable DyeCycling on regular coverslips – instead of the zero-mode waveguides that we use so far. We discuss the pros & cons of a range of fluorogenic systems in the context of #DyeCycling and #smFRET experiments.

Comments welcome!

Our Perspective article where @scisonja.bsky.social and I discuss the potential of #fluorogenic systems for #DyeCycling in #smFRET is now out in QRB Dicovery.

Link: doi.org/10.1017/qrd....