Oscar Wilkins

Muscle MRI quantifies disease progression in amyotrophic lateral sclerosis Background and objectives Quantitative and operator-independent biomarkers of disease progression are urgently needed in amyotrophic lateral sclerosis (ALS) research. We assess the potential of skelet...

Something different from the lab!
We need better ALS biomarkers to minimise size, duration and cost of trials. Muscle MRI could work as it reliably detects progressive atrophy, and correlates with muscle strength loss.
@uclqsion.bsky.social @uclbrainscience.bsky.social
jnnp.bmj.com/content/earl...

06.04.2025 10:10 — 👍 9    🔁 4    💬 1    📌 0

x-axis is time after transformation - was re-transforming a plasmid for this test

12.02.2025 11:49 — 👍 2    🔁 0    💬 0    📌 0

PSA for anyone interested in growing E. coli for plasmid preps in 96 wells. (this is 1 ml of NEB Stable at 37C 240 RPM or 30C 180 RPM). Using TB enables you to obtain as much DNA as a standard miniprep, beautiful

12.02.2025 11:34 — 👍 4    🔁 0    💬 1    📌 0

If you tell me the sequence, I can almost certainly make it, even if my method is different

30.01.2025 18:13 — 👍 5    🔁 0    💬 0    📌 0

Can we make it a rule that when people publish papers involving generation of new plasmids, they put the plasmid sequence in the supplementary? Much more useful than "we digested plasmid A with enzyme B then ligated to C then digested with D then gel purified etc etc etc"

30.01.2025 18:13 — 👍 17    🔁 3    💬 2    📌 3

We're so pleased that groups have been ordering our TDP-REG vectors from Addgene! Please be aware that splicing minigenes cannot easily be used with lentiviruses, because the lentiviral RNA can get spliced before viral packaging! Better to use piggybac or similar, AAV, or just transient transfection

08.01.2025 08:26 — 👍 5    🔁 2    💬 0    📌 1

Glad I joined this platform so I don't miss good quality content like this

28.09.2023 05:37 — 👍 0    🔁 0    💬 1    📌 0