Yes, the buffer that comes with Terra polymerase is always the limiting factor. We have a massive excess of Terra polymerase itself, but unfortunately it doesn’t seem to work with our regular PCR buffer! Would love to know if anyone has managed to get it working well in another buffer!

We have had multiple discussions about why people have not previously observed the very dramatic reduction in transcription upon PNUTS depletion. So I looked at our own TT-seq data and analysed it without including the spike-in. The numbers speak for themselves! Spike-in calibration is important!

We don’t think the impact factor is a good thing. But this is not the fight we want right now. We are trying something amazing and it’s working. We don’t want authors or other journals to fear trying it. Clarivate know they have this control and are using it to squash innovation. It is bullying.

The PNUTS phosphatase complex controls transcription pause release Kelley et al. discover that the PNUTS phosphatase complex plays an essential role in gene transcription by controlling RNA polymerase II pause release. PNUTS achieves this through its TND, which recog...

Latest from the Klose lab: we discover a new role for the PNUTS-PP1 phosphatase complex in regulation of PolII pause release. www.cell.com/molecular-ce...

Fantastic to see the latest story from the Klose lab out in Molecular Cell today! Thoroughly enjoyed being part of a team effort spearheaded by @jesskelley.bsky.social and @edimitrova.bsky.social! A project that briefly dragged us away from CpG islands before leading us back to the 5’ end of genes!