Matthias Böddener

Hello,
do you have a protocol for this? That sounds very interesting.

Alzheimer’s disease features amyloid-beta (Abeta) plaques, ApoE-driven amyloid buildup, and Caveolin1-modulated APP processing. IBA1 marks activated microglia clustering near plaques, fueling immune and inflammatory responses that accelerate neurodegeneration.
#FluorescenceFriday
#Neuroscience

Indirect immunostaining of astrocytes surrounding a blood vessel in the mouse brain with GFAP 🟠. Nuclei have been visualized by DAPI staining 🔵.
#bloodbrainbarrier
#FluorescenceFriday

Ask for test samples from Synaptic Systems. Then you can see how well the cFOS and Arc antibodies work or don't work for your applications. They have a large number of different species.

Ihr begeistert mich immer wieder. Wie man sich so ein Ding ausdenken kann. Sensationell 👍🏻

Indirect immunostaining of a mouse brain section with Aquaporin4 🔴 and Occludin 🟢. Nuclei have been visualized by DAPI staining 🔵.

#bloodbrainbarrier
#FluorescenceFriday

Thanks for your reply.
They are very nice pictures.

Is there an antibody staining in the red blood cells?

Sure, it's from SySy
sysy.com/product/278102

Immunostaining of a mouse brain (hippocampus) section with GluT1 🟣 and ALDH1L1 🟢. Nuclei have been visualized by DAPI staining 🔵.

#bloodbrainbarrier
#FluorescenceFriday

Immunostaining of a Alzheimer's disease mouse brain with Aquaporin 4, P2Y12, APP and Abeta.

Immunostaining of a Alzheimer's disease mouse brain with Aquaporin 4 🔴, P2Y12 🟢, APP 🟡 and Abeta 🟣.

#FluorescenceFriday
#neuroscience

Immunostaining of a rat brain cortex with IBA1 🔴. Nuclei have been visualized by DAPI staining 🔵.
#FluorescenceFriday

Immunostaining of a mouse brain cortex with von Willebrand factor ⚪ and Caveolin1 🔵. Nuclei have been visualized by DAPI staining 🟣.

#bloodbrainbarrier #FluorescenceFriday

Immunostaining of a mouse brain with ATP13A5 and GFAP. Nuclei have been visualized by DAPI staining.

Immunostaining of a mouse brain with ATP13A5 (red) and GFAP (cyan). Nuclei have been visualized by DAPI staining (blue).

#bloodbrainbarrier
#FluorescenceFriday
#Pericytes

Indirect immunostaining of rat hippocampus neurons with
β-Actin (red), Giantin (green) and α-Tubulin (blue). Nuclei have been visualized by DAPI staining (cyan).​

#FluorescenceFriday
#neuroscience
#CellBiology

Have you asked the support team for advice? Our support department can help you with the antibody problems.

Immunostaining of a mouse brain blood vessel with Caveolin1 🔴 and Aquaporin4 🟢. Nuclei have been visualized by DAPI staining 🔵.

#bloodbrainbarrier
#FluorescenceFriday

Mouse retina staining with Kir4.1 Prox1 Calbindin D28k Nuclei

Mouse retina staining with
Kir4.1 ⚪
Prox1🔴
Calbindin D28k🟢
Nuclei 🔵

#bloodretinalbarrier
#FluorescenceFriday

Indirect immunostaining of formaldehyde fixed rat hippocampus neuron with Somatostatin-28 and MAP2. Nuclei have been visualized by DAPI .​

A new #FluorescenceFriday picture.
Indirect immunostaining of a rat hippocampus neuron with Somatostatin-28 🔴 and MAP2🟢. Nuclei have been visualized by DAPI 🔵.​

Please add me, thanks!

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There are lots of us missing, but so many joining so quickly

Please do share, and comment below to be added

go.bsky.app/5f2V6L6

(microglia starter pack also here by @mancusorenzo.bsky.social - bsky.app/starter-pack...)

Thanks 🙂

Please add me

Thanks!

Could you please add me?

Immunostaining of a mouse brain blood vessel with Aquaporin4 and Occludin. Nuclei have been visualized by DAPI staining. #FluorescenceFriday #bloodbrainbarrier

Immunostaining of a mouse brain blood vessel with Aquaporin4 🔴 and Occludin 🟢. Nuclei have been visualized by DAPI staining 🔵.
#FluorescenceFriday #bloodbrainbarrier

Immunostaining of a mouse brain blood vessel.

Immunostaining of a mouse brain blood vessel with Collagen type 4 (cyan) and GFAP (orange) Nuclei have been visualized by DAPI staining (blue).
#FluorescenceFriday
#bloodbrainbarrier

L-type amino transporter1 🔴
Aquaporin4 🟢
DAPI🔵