Rajiv K. Khajuria

🚨 new glioblastoma preprint alert!

we present the first spatially resolved single cell atlas comparing radionecrotic changes (RN) and recurrent IDH-wildtype glioblastoma (GB) –– shedding light on a long-standing diagnostic challenge.

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The immunology of brain tumors The cellular and molecular determinants of the antigenicity of brain tumors and the immunosuppressive brain tumor microenvironment are reviewed.

The immunology of brain tumors

www.science.org/doi/10.1126/...

Childhood brain tumours instruct cranial haematopoiesis and immunotolerance Recent research has revealed a remarkable role for immunosurveillance in healthy and diseased brains, dispelling the notion that this organ is a passive immune-privileged site (1–3). Better understand...

www.biorxiv.org/content/10.1...

Genomic landscape and immunological profile of glioblastoma in East Asian patients Zhong et al. analyze the largest East Asian glioblastoma (EAS-GBM) cohort, identifying four transcriptomic subtypes and distinct genomic features compared to European cases. Notably, EAS-GBMs lack EGF...

Genomic landscape and immunological profile of glioblastoma in East Asian patients

www.cell.com/cell-reports...

🔥 Our second preprint, led by Josch, introduces a new framework to measure mtDNA mutational burden & constraint at single-cell resolution.
We present:
🔹 scmtMPM (mutations per Mb)
🔹 scwMSS (heteroplasmy-weighted local constraint)
👉 www.researchsquare.com/article/rs-6...

Cryo-mtscATAC-seq for single-cell mitochondrial DNA genotyping and clonal tracing in archived human tissues High-throughput clonal tracing of primary human samples relies on naturally occurring barcodes, such as somatic mitochondrial DNA (mtDNA) mutations detected via single-cell ATAC-seq (mtscATAC-seq). Fr...

🚨 New preprints from our lab!
First, we introduce Cryo-mtscATAC-seq, led by Maren (@ms-maren.bsky.social ), enabling high-throughput clonal tracing from frozen human samples by isolating nuclei with their mitochondria (“CryoCells”).
👉 www.biorxiv.org/content/10.1...

EANS-EANO Guidelines on the extent of resection in gliomas Abstract. Intense efforts are employed to optimize neurosurgical resection of gliomas in different clinical scenarios. However, the level of evidence emplo

EANS-EANO Guidelines on the extent of resection in gliomas

doi.org/10.1093/neuo...

Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth The metabolic hallmarks of high-grade glioma (HGG) are not fully understood. Human brain tissue metabolomics revealed that the creatine synthesis pathway intermediate guanidinoacetate (GAA) accumulate...

Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growth

www.biorxiv.org/content/10.1...

Gain-of-function mutant p53 regulates long-noncoding RNA LINC00643 to modulate HIF1α in glioblastoma Gain-of-function mutations of p53 (GOF-MUT-p53) act as oncogenes by regulating gene transcription. We screened for the genome-wide transcriptional targets of GOF-MUT-p53 in glioblastoma (GBM) and foun...

Gain-of-function mutant p53 regulates long-noncoding RNA LINC00643 to modulate HIF1α in glioblastoma

www.biorxiv.org/content/10.1...

Restraint of cancer cell plasticity by spatial homotypic clustering Migliozzi et al. use single-cell resolution spatial transcriptomics of 16 glioblastoma and 2.8 million cells to study tumor architecture. They discover that cell state coherence is maximal in cells or...

Restraint of cancer cell plasticity by spatial homotypic clustering: Cancer Cell

www.cell.com/cancer-cell/...

Integrative multi-omics combined with functional pharmacological profiling in patient-derived organoids identifies personalized therapeutic vulnerabilities of adult high-grade gliomas Background: Precision medicine has transformed cancer treatment by tailoring therapies to specific molecular aberrations. Integrating high-resolution multi-omics with high-throughput functional profil...

Integrative multi-omics combined with functional pharmacological profiling in patient-derived organoids identifies personalized therapeutic vulnerabilities of adult high-grade gliomas

www.biorxiv.org/content/10.1...

Protein-based classification reveals an immune-hot subtype in IDH mutant astrocytoma with worse prognosis Tang et al. apply spatiotemporal multi-omics to define four proteomic subtypes of IDH-mutant astrocytoma. They highlight an immune/mesenchymal-enriched (IME) subtype marked by gemistocytic differentia...

Protein-based classification reveals an immune-hot subtype in IDH mutant astrocytoma with worse prognosis

www.cell.com/cancer-cell/...