KPL ApS

Introducing Trypsin RMS – our third product is here.

Recombinant. Methylated. Sequencing-grade.

The robust trypsin #proteomics labs need, without the batch variability of animal-derived alternatives.
Learn more: kplbio.com/trypsin/

#TeamMassSpec #science #proteins #enzymes

References:
Graf L & Szilágyi L (2003) "Trypsin: is there anything new under the Sun?" J Mol Struct (Theochem) www.sciencedirect.com/science/arti...
en.wikipedia.org/wiki/Trypsin
en.wikipedia.org/wiki/Wilhelm...

The answer is 🤭 , 1876!

Wilhelm Kühne discovered trypsin at the University of Heidelberg. He also coined the term "enzyme" the following year.

"Trypsin" comes from the Greek word "thrýpto" meaning "I break".

150 years later it's the most used protease in mass spec for breaking ... ahem proteins!

Trypsin is the workhorse of bottom-up #proteomics. But when was it first discovered? Lets try an emoji poll !

😇 1836
🤭 1876
😜 1897
🤑 1931

Reply with your guess!

Correct answer in the replies!

#TeamMassSpec

Comparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows This study presents a comparative analysis of three LysC endopeptidase homologues from Achromobacter lyticus (A. lyticus),Pseudomonas aeruginosa and Lysobacter enzymogenes for mass spectrometry-based proteomics. Utilizing a protein aggregation capture workflow with HeLa cell lysates, we assessed the enzymes’ cleavage specificity, digestion efficiency, and performance across various experimental conditions. Results showed that while all three LysC homologues exhibited high cleavage specificity at lysine residues, A. lyticus LysC outperformed the two others with superior peptide identification, digestion efficiency, and protein coverage, especially at shorter digestion times. Our experiments using a combination ofA. lyticusLysC and trypsin demonstrated the importance of employing LysC for significantly minimizing missed cleavage rates in tryptic digests, especially with regard to lysine-containing peptides. This study underscores A. lyticus LysC’s potential as an optimal choice for enhancing mass spectrometry-based proteomics.

Does enzyme quality matter in #proteomics?

New study in JPR w/ @jesperolsenlab.bsky.social shows: LysC-Trypsin reduces missed cleavages from 30% → 15%

KPL's A. lyticus LysC achieves >90% efficiency in systematic comparison.

Congrats to the authors!
pubs.acs.org/doi/10.1021/...
#TeamMassSpec

Happy Holidays from KPL! 🎄

Our first full year: 2 product launches, lots of foundation building, and countless hours pushing #proteomics forward.

Our commitment: high quality, high performance, competitive prices. No compromises.

2026 is looking exciting - stay tuned! ✨

From the proteomics community on Reddit Explore this post and more from the proteomics community

Best method for RELATIVE protein abundance from #proteomics experiments?? 🤔🤔🤔

#TeamMassSpec

www.reddit.com/r/proteomics...

From the proteomics community on Reddit Explore this post and more from the proteomics community

#proteomics choose your fighter!

www.reddit.com/r/proteomics...

🚀 Our very own @tsbatth.bsky.social & others not on Bluesky will be at Nordic Conference in Mass Spectrometry & Proteomics 2025!

📅 Sept 1-3 | 📍Odense, Denmark 🔬Presenting the latest protease innovations!

Posters on Sept 1 and 2!

kplbio.com/kpl-aps-to-u...

#MSP2025 #Proteomics #TeamMassSpec

Addressing NHS chemistry: Efficient quenching of excess TMT reagent and reversing TMT-over labelling in proteomic samples by methylamine N-Hydroxysuccinimide (NHS) ester chemistry is used extensively across proteomics sample preparation. One of its increasingly prevalent applications is in isobaric reagent-based quantitation such as iT...

A basic solution to a very common problem.

collab with @kusterlab.bsky.social and @bendavisgroup.bsky.social

www.mcponline.org/article/S153...

Shout out to the organisers @jesperolsenlab.bsky.social, (Tina Ludwig) @baybioms.bsky.social kathrynslilley.bsky.social, Caroline May and Britta Eggers
and the speakers @bonalditiziana.bsky.social, @coscialab.bsky.social, @kusterlab.bsky.social, @anamdv.bsky.social, @jsandow.bsky.social

Attention Skyline Users? Going to ASMS and have an interesting application of Skyline or Panorama? March 14th is the abstract deadline for the Skyline User Group Meeting. skyline.ms/project/home...
#proteomicsky #massspecsky #massspec

❄️ WINTER SALE ALERT! ❄️ Big savings on our products! Cozy deals that'll warm your heart and your wallet. 🔥 Shop now before these cool prices melt away! Find out more here:
kplbio.com/product/lysc/
kplbio.com/product/arg-c/
#proteomics #teammassspec

🎄 Wrapping up a amazing year at KPL! ✨As we celebrate this season, we're grateful for our wonderful customers and team. 🌟We look forward to making 2025 even more extraordinary! 🎉 Happy Holidays from our family to yours! ⭐️ #HappyHolidays #NewYear2025 #proteomics #TeamMassSpec

#DAPSOC CelebrateMS is underway, starting with the honoring of Peter Roepstorff's contributions to #Proteomics and #TeamMassSpec . He made a surprise appearance to standing ovation!

Analysis of FAIMS for the Study of Affinity-Purified Protein Complexes Using the Orbitrap Ascend Tribrid Mass Spectrometer www.biorxiv.org/cont...

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#proteomics #prot-preprint

🧬🔬 KPL ApS at DAPSOC 2024!
🎉 Celebrating 50 years of mass spectrometry in Denmark

Our very own @tsbatth.bsky.social will be presenting our latest research

🚀 Join us Dec 3-4 at SDU Odense! 🧪
#DAPSOC2024 #Proteomics #TeamMassSpec

Arg-C Zero - High-Fidelity Protease for Mass Spectrometry | KPL Bio Discover Arg-C Zero [KPL0146], a high-fidelity arginyl endopeptidase delivering 0% missed cleavages. Ideal for proteomics, Arg-C Zero ensures complete digestion, high purity, and compatibility with ma...

🚀Introducing Arg-C Zero (KPL0146)🧬

Next-gen proteomic enzyme with close to 0% missed cleavages at Arginine residues!

🔬100% Arginine cleavage + ultimate specificity = next-level protein analysis magic ✨

🧬 Details: kplbio.com/product/arg-c/

#Proteomics #TeamMassSpec #proteins

Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays An approachable framework for the scalable implementation of proteome-wide thermal shift assays to assess drug mechanisms of action.

That’s a wrap, we’ve uploaded our version of record for the large scale PISA work led be Jon & co. to @elife.bsky.social :
elifesciences.org/articles/95595

Thanks to the reviewers for helping us improve the manuscript, enjoyed the process of developing this resource.

🧪 The Scientists in Proteomics starter pack has doubled in size this week.

go.bsky.app/3XETWrh

Advancements in mass spectrometry-based proteomics: a new era in pathology research and diagnostics link.springer.com/ar...



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#proteomics #prot-paper

Excited to join the Bluesky community!