Bert van de Kooij

PhD student DNA repair in kidney cancer | Werken bij UMC Groningen

We have an open position for a PhD student to join our team. If you have an interest in cancer biology, genome maintenance, or both, please apply! Details can be found in the link below. With @marcelvanvugt.bsky.social.

werkenbijumcg.nl/-/vacatures/...

Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks - Nature Communications Correct repair of broken DNA molecules is required to prevent potentially oncogenic mutations. To study repair fidelity and mechanism, van de Kooij et al. developed single cell reporters that detect i...

Interesting read. Aligns well with our findings (doi.org/10.1038/s414..., together with @haicovanattikum.bsky.social). We found DNA-PKcs inhibition to increase Cas9 break repair by single strand annealing (SSA), causing kilobase sized deletions. Exo1 depletion prevented this increase in SSA.