Mono-ADP-ribosylation as a degradation mark?
@andriigorelik.bsky.social, Ivan Ahel et al observe increased ADP-ribosylation of aryl hydrocarbon receptor (AHR) & PARP7 upon ubiquitin-proteasome blockage, crosstalk with DTX2 E3, as rapid AHR-transcription off-switch
www.embopress.org/doi/full/10....

Ubiquitin pathway blockade reveals endogenous ADP-ribosylation marking PARP7 and AHR for degradation | The EMBO Journal imageimageDegradation mechanisms of transcriptionally active aryl hydrocarbon receptor (AHR) are unclear. This work reveals that PARP7 ADP-ribosylates itself and ligand-bound AHR, creating a recogniti...

The interplay between ADP-ribosylation and ubiquitination, mediated by PARP7 and DTX2 proteins, can serve as a rapid AHR transcription “off-switch” through proteasomal degradation.
www.embopress.org/doi/full/10....

Akin to bacterial SIR2 antiphage proteins, human SIRal, also known as FAM118b, forms filaments that are essential for its NAD processing activity.

Awesome to see structures of these filaments that differ from bacterial ones.

Congrats to the authors on this beautiful study.

Filament formation and NAD processing by noncanonical human FAM118 sirtuins Nature Structural & Molecular Biology - Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that...

Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.

Filament formation and NAD processing by noncanonical human FAM118 sirtuins - Nature Structural & Molecular Biology Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that FAM118A/B processing of NAD involves head-to-tail filament...

Please check our collaborative paper with Marcin Suskiewicz and Sebastien Huet labs on FAM118 proteins – non-canonical sirtuins that form filaments and act as NADases in human and other vertebrate cells.
www.nature.com/articles/s41...

Histone PARylation factor 1: a review of its role in the DNA damage response Abstract. Although poly-(ADP ribose) polymerase 1 (PARP1) and PARylation of histones have been known for over 50 years and have been successfully targeted

Beautiful review from J. Rudolph on Histone PARylation factor 1: a review of its role in the DNA damage response url: academic.oup.com/nar/article/...

Converting a kinase through molecular mimicry - Dunn School Published in EMBO Reports, a new study by the Thurston Group reveals how a small virulence protein from Salmonella called SteE, uses molecular mimicry to reprogramme host kinase specificity.

A new study by @thurstonlab.bsky.social reveals how a small virulence protein from Salmonella uses molecular mimicry to reprogramme host kinase specificity.

Find out more: www.path.ox.ac.uk/news-article...

Here you can read our full review paper on the interplay between ADP-ribosylation and ubiquitination, with special focus on dual, hybrid modification ADPr-Ub:
pubmed.ncbi.nlm.nih.gov/41065402/

This News & Views article provides a nice overview of the recent discoveries that RNF114 recognizes a dual modification ADP-ribose–ubiquitin (ADPr-Ub) and subsequently extends it by adding additional ubiquitin units.:
pubmed.ncbi.nlm.nih.gov/41039156/

RNF114 and RNF166 exemplify a new E3 ligases family that recognizes #MARUbylation –hybrid ADP-ribose/ubiquitin modifications– and extends them with K11-linked polyubiquitin
@jnpruneda.bsky.social @michaelnadbio.bsky.social and colleagues
www.embopress.org/doi/full/10....

The September @natsmb.nature.com issue is out! www.nature.com/articles/s41...
We feature very interesting papers on ubiquitylation and degradation. Really excited to continue to serve the proteostasis and PTMs communities and publish conceptually insightful work.

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - Nature Biotechnology Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - @helmholtz-hiri.bsky.social go.nature.com/4n7zDpG

Applications now open for our PhD studentship programme - Dunn School Applications are now open to apply for our four year, fully-funded PhD programme in Molecular Cell Biology in Health and Disease.

Our PhD studentship programme is officially open; pass it on

If you are looking for a fully-funded PhD in the mechanisms underlying human disease, here are the details...

Great science and a great environment!

www.path.ox.ac.uk/news-article...

The rise of ADP-ribose–ubiquitin Nature Structural & Molecular Biology - Post-translational modifications show mechanistic crosstalk, exemplified by the ADP-ribose–ubiquitin hybrid signal, in which one post-translational...

A few years back we discovered a dual hybrid protein modification composed of an ADP-ribose dinucleotide and the ubiquitin moieties (ADPr-Ub). Here you can read our review that will give you an update on this increasingly popular topic:
rdcu.be/eETIT

We have postdoc positions available to study roles of ADP-ribosylation and ubiquitination signalling in genome stability, infection or immunity. Please enquire if you are interested.

📣 Applications now open 📣

We are seeking outstanding ECRs looking for a stimulating and supportive environment in which to establish their research group as externally-funded fellows, in any area or cell or molecular biology underlying disease!

Please share!

www.path.ox.ac.uk/work-with-us...

Postdoctoral Fellow - Costa Lab Salary for this Role: From £45,500 with benefits, subject to skills and experience. Job Title: Postdoctoral Fellow - Costa Lab Reports to: Alessandro Costa Closing Date: 03/Aug/2025 23.59 GMT Job Desc...

Less than two weeks left to apply!

Postdoc positions in my lab to study

1. initiation of DNA replication.

2. chromatin replication/epigenetic inheritance.

Great for biochemists, biophysicists & structural biologists.

Deadline 3 August 2025.

crick.wd3.myworkdayjobs.com/External/job...

The application deadline has been extended for a postdoc position in our lab until August 22. Please enquire/apply if you are interested in ADP-ribosylation, ubiquitination, genome stability or immunity.
my.corehr.com/pls/uoxrecru...

Colin Kleanthous' fundraiser for Motor Neurone Disease Association Help Colin Kleanthous raise money to support Motor Neurone Disease Association

My wonderful colleage Colin Kleanthous is raising money for the MND association. Please support Colin in this worthy endeavor! www.justgiving.com/page/colin-k...
(Please repost!)

Regulation of a phage defence island by RptR, a novel repressor that controls restriction–modification systems in diverse bacteria Abstract. Bacteria encode a panoply of defence systems to overcome phage infection. In recent years, over 100 defence systems have been identified, with th

Defence island in Escherichia coli composed of DarTG toxin-antitoxin system embedded within a Type I restriction-modification system is regulated by the RptR (RMS-proximal transcriptional regulator).
academic.oup.com/nar/article/...

Identification of RNF114 as ADPr-Ub reader through non-hydrolysable ubiquitinated ADP-ribose - Nature Communications Deltex E3s modify ADP-ribosylated targets with ubiquitin, creating a hybrid modification whose readers remains unknown. Here, the authors synthesise a non-hydrolysable probe that mimics the modificati...

A few years back we discovered a dual hybrid protein modification composed of ADP-ribose dinucleotide and ubiquitin (ADPr-Ub). Now we reveal that ADPr-Ub can be further ubiquitinated by the E3 ubiquitin ligase RNF114!
www.nature.com/articles/s41...

www.science.org/doi/10.1126/...

Our review article 'PARPs and ADP-ribosyl hydrolases in cancer therapy: from drug targets to biomarkers' is now available online.
doi.org/10.1016/j.dn...

We have a postdoc position available to work on interplay between ADP-ribosylation and ubiquitination in regulation of genome stability and/or immunity. Expertise in cell biology is required.
my.corehr.com/pls/uoxrecru...

New paper! We uncover mechanistic insights into the crosstalk between AHR (aryl hydrocarbon receptor, a transcription factor) and PARP7 (a nuclear enzyme that performs protein ADP-ribosylation). Both are promising cancer drug targets.

Big thanks to all co-authors!

www.pnas.org/doi/10.1073/...

CRISPR screens and quantitative proteomics reveal remodeling of the aryl hydrocarbon receptor–driven proteome through PARP7 activity | PNAS PARP7 is an enzyme that uses donor substrate NAD+ to attach a single ADP-ribose moiety onto proteins related to immunity, transcription, and cell g...

Our new paper is out! Trying to understand the molecular function of the PARP7, a PARP family enzyme with links to innate immunity and environmental toxins response. Congrats to Andrii and the rest of the team and collaborators.
www.pnas.org/doi/10.1073/...

We have another postdoc position available to work on interplay between ADP-ribosylation and ubiquitination in regulation of genome stability. For this post expertise in cell biology is required.
my.corehr.com/pls/uoxrecru...

We have another postdoc position available to work on interplay between ADP-ribosylation and ubiquitination in regulation of genome stability. For this post expertise in cell biology is required.

my.corehr.com/pls/uoxrecru...

⏰ Deadline approaching for postdoc position in my lab, Dunn School, University of Oxford.

We work on how the cell's antenna receives and transmits essential signals through motor protein driven transport.

❄️ Cryo-EM/ET, smTIRF, cell biology methods 🧪🔬

30/5/25 ➡️ tinyurl.com/KToropovaPDRA

Two year postdoc position available in the Sparte lab at IGDR to investigate the crosstalk between ADP-ribosylation and SUMOylation signaling at stressed replication forks

Happy that my work on RNF213 has been published as a part of doi.org/10.1038/s414...!

Many thanks to all the groups involved:
@clausenlab.bsky.social
@e3chembio.bsky.social
@fletcherlab.bsky.social

Have a look at the summary from Fletcher lab for an insider perspective of the paper's history: