Erwin Schoof

That was a later upgrade as projects got bigger and bigger - long live the RAID cards 😅

We built a custom Thread ripper (7980X) rig with 256Gb of RAM and 24Tb of SSD to plough through all the single-cell raw files. Up-front investment, but cheaper in the long run compared to paying for CPU time on an HPC. And it's FAST, runs my whole lab! 😅

Very cool stuff indeed Luke, congrats!

Pumped to finally show off our spatial proteomics work presented a few weeks ago at the EAS Congress @eassociety.bsky.social and now live on @biorxivpreprint.bsky.social 🥳

Time for a science thread! #TeamMassSpec #SpatialProteomics #AtheroSky 🧵

www.biorxiv.org/content/10.1...

Tried presenting my data at #EuPA2025. Then decided to scale things up... Turns out, volcano plots look great in sand and people are more interested in what we scientists do.
#Proteomics #YPIC #YPIContest2025

The reports of Bluesky’s death have been greatly exaggerated

The financial crisis of the HE sector in the UK now leads to increased numbers of grant and fellowship applications, pushing down the success rates. Since also overheads increased, in some cases we will see success rates halved.

There is a BBSRC fellowship which will have a success rate of 5%.

It is so crazy! Not that I have answers, but more money is clearly needed to bring funding rates back to 15-20% to ensure all the great ideas get to be pursued. And this problem is unfortunately not limited to the UK; a Danish call earlier this year only funded 4 out of 77 applications-> that's 5%!

#ThermoFisher #IonOpticks #EvoSep #Cellenion #Biognosys

And last but not least, a big shout out to our valuable tech partners, without whom we couldn't do what we really want: namely making a difference in the world with biological discoveries through single-cell proteomics by Mass Spectrometry

Another big shout out to the organizers of #EuPA2025 who allowed me to share our recent results with the community, and who did a fantastic job putting together such a great conference of nearly 500 people in a location most of us only dream about visiting.

Big shout out to my lab who enabled me to present some of the cool stuff they spend their valuable energy on, to #DTUBioengineering for hosting our efforts, and my funders for funding our work.

On my way home from what was an absolute blast of a EuPA2025 in the lovely Saint-Malo in France. So much great science, amazing people, old & new friends, and not the least, our valuable tech partners. The future of proteomics is clearly still only beginning & we all seem to be enjoying the ride! :)

Thanks for the share and update - we certainly benefit from all the amazing tech in scp-MS!

The year of "How deep can we go?"
Erwin Schoof presents a view of the deepest proteome coverage across various cell types at #EuPA2025, using the newly released Orbitrap Astral Zoom. Analysing 40 HEK2983T cells per day, using an Aurora Ultimate 25x75 will consistently get you ~6,000 protein IDs.

Ok thanks, please do keep us posted if you hear anything, as will I. Nice to know it's not a hard "no" at least!

Well done buddy, fingers crossed! 🤞🏻

So does that mean you actually have it working reproducibly on your OT-2? Ours never worked well unfortunately, so we're also still stuck with spinning, and a bit annoyed when we found out the Flex head doesn't interface with EvoTips - if you hear more about the Flex work, that would be amazing :)

Our amazing group of experts: Jenn Abelin, @berndwollscheid.bsky.social, @erwinschoof.bsky.social , Tim Van Den Bossche and Stefanie Hauck 👏
If you would like to join, please register using the QR code or this link: docs.google.com/forms/d/e/1F...
#Proteomics #EuPAFPS2025 #EuPA2025 #SaintMalo

Still figuring out which evening workshop to attend tonight at #ASMS2025? Come on by Ballroom II and hear about hands-on tips and tricks for one of the hottest and fastest developing areas within LC-MS based proteomics, namely single-cell. 😃

Tenure-track Research Group leader position in translational cancer research at the Finsen Laboratory, Rigshospitalet, Copenhagen University Hospital A group leader position at the Finsen Laboratory, Rigshospitalet, Copenhagen University Hospital is open for appointment as soon as possible. The position is av

WE ARE RECRUITING: A tenure-track group leader position in translational cancer research is available at the Finsen Laboratory in Copenhagen. Come join an excellent and friendly research environment combining both hospital and university affiliated groups

candidate.hr-manager.net/ApplicationI...

#ASMS2025 offers great evening workshops. If you are interested in real-time decision-making during MS data acquisition or still looking for one to join,

consider the Real time Mass Spectrometry Workshop featuring speakers and panelists Sarah Sipe, Aarthie Senathirajah, and Manuel Peris Diaz.

#ASMS2025
Come here about the latest in real-time instrument control including applications to single cell proteomics (@parallelsq.bsky.social), #glycotime, and quantitative proteomics!

Integrating Alternative Fragmentation Techniques into Standard LC-MS Workflows Using a Single Deep Learning Model Enhances Proteome Coverage We built and characterised a mass spectrometer capable of performing CID (both beam type and resonant type), UVPD, EID and ECD in an automated fashion during an LCMS type experiment. We exploited this...

I am really pleased we managed to squeeze this story out during #ASMS2025. This is our contribution relating to the fantastic Omnitrap/Orbitrap. What we thought would be a straightforward conversation of our exploris-omnitrap turned into quite a journey. (1/n)

www.biorxiv.org/cgi/content/...

Evaluation of an Orbitrap Astral Zoom mass spectrometer prototype for quantitative proteomics - beyond identification lists Mass spectrometry instrumentation continues to evolve rapidly, yet quantifying these advances beyond conventional peptide and protein detections remains challenging. Here, we evaluate a modified Orbit...

New Preprint ... This was a bit different of a project than what we normally do for hardware comparisons. I think there is a lot of potential in the methods mentioned here for calibrating the instrument response to a common scale. Nice work by @chrhsu.bsky.social!

www.biorxiv.org/content/10.1...

Evaluation of an Orbitrap Astral Zoom mass spectrometer prototype for quantitative proteomics - beyond identification lists www.biorxiv.org/cont...

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#proteomics #prot-preprint

Yesterday, we fired up the grill at our annual Evosep User Meeting. It was inspiring to reconnect with users and colleagues from the #proteomics community.

If you’ve got room for a little more orange in your morning, join us for breakfast tomorrow. www.evosep.com/asms2025/

Thanks for the kind shout out, Ben! 🙏🏻 Hope to catch you in Baltimore!

Super excited to have this work out & share it at large with the community at #ASMS2025. Jakob came up with a really clever way to integrate targeted&global proteomics, and together with the @porsebo.bsky.social lab, we pushed the boundaries of what we thought biologically possible. Congrats Jakob!🥳

#ASMS2025