Christian Wunder @ Curie Institute

Video snippet courtesy of Belfast legend David Holmes on Instagram...

22.02.2025 20:04 — 👍 14602    🔁 4096    💬 200    📌 178

Spatial N-glycan rearrangement on α5β1 integrin nucleates galectin-3 oligomers to determine endocytic fate Membrane glycoproteins frequently adopt different conformations when altering between active and inactive states. Here, we discover a molecular switch that exploits dynamic spatial rearrangements of N...

... and soon the structural glycoswitch: www.biorxiv.org/content/10.1...

22.02.2025 22:31 — 👍 4    🔁 2    💬 0    📌 0

"Growth factor-triggered de-sialylation controls glycolipid-lectin-driven endocytosis" An excellent collaboration between the US, India, Denmark, UK, Netherlands and France.

Growth factors trigger receptor desialylation at the plasma membrane, galectin binding and subsequent endocytosis.

=> A proton pump driven glycoswitch @naturecellbiology.bsky.social :

rdcu.be/eaLM9

#glycotime, @institutcurie.bsky.social, @cnrs.fr, @inserm.fr, #NIH, #CellBiol, #Cancer

22.02.2025 22:31 — 👍 16    🔁 3    💬 2    📌 1

Happy to contribute to the analysis of β1integrin endocytosis using @the.3i.social LLSM, now in @naturecellbiology.bsky.social
rdcu.be/eaLM9
L. Leconte @christianwunder.bsky.social @aforrester.bsky.social @ewanmacdonald.bsky.social @institutcurie.bsky.social #inria @france-bioimaging.bsky.social

22.02.2025 09:01 — 👍 13    🔁 6    💬 0    📌 2

Lipid-directed covalent fluorescent labeling of plasma membranes for long-term imaging, barcoding and manipulation of cells Fluorescent probes for cell plasma membrane (PM) are generally based on amphiphilic anchors that incorporate non-covalently into biomembranes. Therefore, they are not compatible with fixation and permeabilization, presence of serum, or cell co-culture because of their exchange with the medium. Here, we report a concept of lipid-directed covalent labeling of PM, which exploits transient binding to lipid membrane surface generating high local dye concentration, thus favoring covalent ligation to random proximal membrane proteins. This concept yielded a class of fluorescent probes for PM (MemGraft), where a cyanine dye (Cy3 and Cy5) bears at its two ends low-affinity membrane anchor and reactive group: an activated ester or a maleimide. We found that MemGraft probes with these reactive groups provide efficient PM labelling, in contrast to a series of control compounds, including commercial Cy3-based labels of amino and thiol groups, revealing the crucial role of the membrane anchor combined with high reactivity of activated ester and a maleimide groups. In contrast to conventional PM probes, based on non-covalent interactions, MemGraft labelling approach is compatible with cell fixation, permeabilization, trypsinization and presence of serum. The latter allows long-term cell tracking and video imaging of cell PM dynamics without signs of phototoxicity. The covalent strategy also enables staining and long-term tracking of co-cultured cells labelled in different colors without probes exchange. Moreover, combination of different ratios of MemGraft-Cy3 and MemGraft-Cy5 probes enabled long-term cell barcoding in at least 5 color codes, important for tracking and visualizing multiple cells populations. Ultimately, we found that MemGraft strategy enables efficient biotinylation of cell surface, opening the path to cell surface engineering and cell manipulation. ### Competing Interest Statement The authors have declared no competing interest.

Interesting ChemRxiv preprint by the group of Andrey Klymchenko. They develop fluorescent probes that enrich in the membrane and then covalently label proteins. This leads to plasma membrane labeling, which is resistant to cell permeabilization. #ChemBio www.biorxiv.org/cont...

28.11.2024 15:00 — 👍 63    🔁 21    💬 2    📌 0

SAVE THE DATE 🗓️Ubiquitin & Friends Symposium: 8-9 May 2025 in Vienna!
Registration will open🔜 Keep an eye out for the announcement 👀
✨guest speaker lineup👇🧵 +talks from abstracts! Great chance for #ECRs to meet with peers & experts in a friendly setting!
🔗 www.protein-degradation.org/symposium/

29.11.2024 14:18 — 👍 60    🔁 28    💬 1    📌 4

We said hello to our new followers last week, but there's many more of you now! Welcome! We've created a starter pack for you, which is a work in progress.

We'd also love to hear from you about your work we've published or what kind of things you want us to post.

12.11.2024 15:43 — 👍 99    🔁 42    💬 16    📌 6

More very cool research from the Hegde Lab! Looking forward to diving in :)

🔗to the original paper out @science.org here: www.science.org/doi/10.1126/...

29.11.2024 09:55 — 👍 13    🔁 2    💬 0    📌 0