HillerLab

Alpha-synuclein interacts with regulators of ATP homeostasis in mitochondria - Nature Communications Here, the authors show that physiological alpha-synuclein supports mitochondrial ATP homeostasis via interactions with ATP synthase and AK2, whereas its disease-linked mutants, truncated forms, and ag...

Excited to play a small role in this great paper: www.nature.com/articles/s41... by providing high quality purified chaperones by@masgu.bsky.social ! Huge congrats to first authors Tetiana Serdiuk and Yanick Fleischmann and the @picottilab.bsky.social and Roland Riek's lab for their work!

We are thankful to our collaborators from the @spanglab.bsky.social and @maierlab.bsky.social @biozentrum.unibas.ch @unibas.ch

These condensates could revolutionize our understanding of protein folding and inspire new therapies for misfolding diseases like diabetes and cancer. A new era for targeting ER function! #Biomedicine #Therapeutics

Surprisingly, our findings reveal that PDIA6 multi-chaperone condensates are critical for insulin secretion by enhancing proinsulin folding, overturning decades-old models of insulin processing.

PDIA6 condensates recruit Hsp70 BiP, ERdj3, and Grp94 creating a functional hub for protein folding. The condensates dissolve upon ER stress but reform after stress relief, regulated by Ca²⁺ levels. This dynamic switch suggests a novel mechanism for maintaining ER homeostasis!

New paper alert! Stellar work by brilliant lead first author @annaleder.bsky.social together with project leader @masgu.bsky.social reveals a groundbreaking discovery: PDIA6 forms multi-chaperone condensates creating "folding factories" in the ER. www.nature.com/articles/s41...

Last day for our summer student Shreyan Datta, who has done great work during his one and a half months in our lab working on the Hsp70 functional cycle! If you are looking for a PhD student in the next few months, Shreyan Datta will be a great fit for any lab!

Predictions of Steady-State Photo-CIDNP Enhancement by Machine Learning Photochemically induced dynamic nuclear polarization (photo-CIDNP) is a hyperpolarization method used to boost signal sensitivity in NMR spectroscopy. So far, there is no theory to predict the steady-...

Congratulations to PhD student Marta and NMR Facility Manager Thomas for the publication of their great work on Photo-CIDNP in JACS ! pubs.acs.org/doi/full/10....

Remember today !

Congratulations to @annaleder.bsky.social receiving the Shepherd price for her PhD work at today’s @biozentrum.unibas.ch ‬ symposium @biozentrum-phd.bsky.social . Stay tuned for the official paper release in the coming weeks.

Thrilled to share our new method in-cyclo NMR that lets us watch molecular machines in action — decoding molecular machine kinetics with atomic precision and exceptional time resolution in one go! 🚀 Check it out: doi.org/10.1038/s414... #NMR #StructuralBiology

Our data reveal that calcium doesn’t affect the cycle, but combined with ADP accumulation it creates off-pathway states that prolong the nucleotide-bound lifetimes. This changes Hsp70’s function extending client protein interaction times — a new layer of cellular regulation!

This versatile in-cyclo NMR platform is adaptable to study diverse ATP-driven molecular machines beyond Hsp70 — enabling precise dissection of functional cycles, allosteric regulation, and the effect of cofactors or co-chaperones at unprecedented resolution.

Applied to the Hsp70 BiP NBD, in-cyclo NMR reveals all 11 microscopic kinetic rates of ATP binding, hydrolysis & product release — showing ADP·Pi release, not ATP hydrolysis, is the rate-limiting step of the cycle duration. Stay tuned for more results on the full-length Hsp70.

Mechanism of ATP hydrolysis in the Hsp70 BiP nucleotide-binding domain - Nature Communications Mas et al introduce in-cyclo NMR to quantify all states and kinetic steps of Hsp70 chaperones ATPase cycle. In-cyclo NMR will enable studies of other molecular machines at an unprecedented level of de...

Introducing in-cyclo NMR, a breakthrough method led by first author @masgu.bsky.social at @biozentrum.unibas.ch! This method combines high-resolution NMR with ATP regeneration & phosphate removal to resolve kinetics & dynamics of molecular machines - all in one experiment! doi.org/10.1038/s414...

Great opportunity to hear how our Ph.D student Marta Stefanska brings light into NMR!

Tired of costly cloning software? Try IVA Prime: a free, game-changing tool for automated primer design in IVA cloning! Simplify complex cloning with one click, reduce errors, and save time. Visit www.ivaprime.com. doi.org/10.1093/nar/... collaboration with Prof. Javier Garcia Nafria

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Great meeting so many inspiring colleagues and friends from the #NMR community at this year’s @enc-conference.org in Asilomar! I rarely travel to the US—trying to keep my carbon footprint low. But how big is the footprint of a #conference like ENC/ISMAR 2025? I analyzed the data. 🧵👇

Amazing opportunity in the group of Prof. Nadinath Nillegoda. A great scientist and group leader!

Check out our latest story on the cryoEM structure of the BAM-SurA holo insertase complex! Out in #ScienceAdvances (www.science.org/doi/full/10....)

Big week ahead for our lab! @annaleder.bsky.social presents at the 1.2 GHz inauguration in Zurich, and @masgu.bsky.social presents at @enc-conference.org (5:05pm PT) in Asilomar #ENCISMAR2025. They’ll share disruptive ideas on enzymology, molecular chaperones, LLPS, and ER biology. Don’t miss it!