RadfordLab

Molecular insights into how the motions of the β-barrel and POTRA domains of BamA are coupled for efficient function Nature Communications - BamA is the catalytic core of the BAM complex, which inserts proteins into bacterial outer membranes. Here, the authors show that hinge flexibility between BamA’s...

See our latest paper on BAM action. Great work to the team and super collaborations with @naranson.bsky.social @antoncalabrese.bsky.social and @Jean Francois collet. Have a read rdcu.be/eJjDp

Employing deep mutational scanning in the Escherichia coli periplasm to decode the thermodynamic landscape for amyloid formation | PNAS Deep mutational scanning (DMS) assays provide a powerful method to generate large-scale datasets essential for advancing AI-driven predictions in b...

Delighted to see our new DMS of amyloid formation and predication of amyloid stability vs sequence changes out at PNAS. Brilliant work Conor! doi.org/10.1073/pnas...

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Our review on CPEB and memory published today- thanks to all authors. Enjoy the read

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Delighted to see Jon Machin's lovely OMP folding and assembly work on the front cover of JMB (doi: 10.1016/j.jmb.2025.169346) and in this Faraday Discussion article (doi: 10.1039/d4fd00180). Congratulations on your great work Jonathan!

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Interested in amyloid polymorphs? Our latest paper with PhD student Jack Connor is just out. Have a read and do try out his analysis on your favourite amyloid structures! doi.org/10.1016/j.st...

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Delighted to share our latest paper on OMPs and the effects of their interactions on OMP catalysis in the bacterial outer membrane. The latest super work from Jonathan Machin. See doi.org/10.1016/j.jm...

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Great day celebrating Katy Dewison’s PhD. Good luck in all you do in the future Katy in your postdoc at Aarhus. We will miss you’

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Super FASEB meeting on protein misfolding and amyloid fibre polymorphs in Phoenix Arizona. Hot science and a hot place (45C)! Katy and Jack did a great job presenting their data in talks and posters.

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So proud our lab was awarded Gold LEAF status for sustainability. Thanks to Nasir Khan, Tabitha Howe and @antoncalabrese.bsky.social for making it happen for us all! Keep up the good work!

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Two prizes in one day! So proud of Maddie Gilbert and Katy Dewison who each was awarded a prize for their PhD thesis work in the Faculty of Biological Sciences on Alzheimer’s and Parkinson’s disease respectively.

Beautiful and inspiring talk by Sheena Radford of the @radford-lab.bsky.social at the Astbury Centre for Structural Molecular Biology (@astbury-bsl.bsky.social) from the University of Leeds (@universityofleeds.bsky.social) at our annual Bijvoet Symposium!

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Honoured to sign the book to become an international member of the National Academy of Sciences. Proud to receive this in recognition of all the work of many group members and collaborators over the years. Thank you all.

Our commentary on flow and amyloid formation published today www.pnas.org/doi/10.1073/pnas.2504573122

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At last our paper is out at JACS and we made the front cover! Thanks to Phospho animation for the image. See doi.org/10.1021/jacs....

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Had a wonderful time at ADAM10 in Gothenburg with old and new friends! Thank you for a great 3 days of amyloid science.

Looking forward #ADAM10 kick-off 🇸🇪 3 days of all things amyloid and great opportunity to meet old friends and making new ones! Thanks @professorpernilla.bsky.social and Elin Esbjorner et al for such a great program. Reach out if you are around
www.chalmers.se/en/conferenc...

Just some of the amazing scientists @universityofleeds.bsky.social in FBS, #international women’s day

Proud to be part of this great article with @ldaubrey.bsky.social . Of interest to all working on amyloid polymorphs see doi.org/10.1016/j.jmb.2025.169008

Delighted our latest paper on amyloid polymorphs is published in JACS- doi 10.1021/jacs.4c16743. Well done all- very proud!

BPS day 2! @emilyjbyrd.bsky.social @radford-lab.bsky.social @biophysicalsoc.bsky.social

Super visit to UTSWCentre for Alzheimer and Neurodegeneration yesterday and to see Nikos Louros- a great host in a frozen and cold Dallas!

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Are you interested in proteins as exciting, dynamic interacting species. Come and join our meeting on March 28th 2025. Places are limited so register ASAP (£25 only!) at sheena-radford-lab.uk/fida.php.

New news- thanks to British Biophys Soc travel bursaries will be available for ECR registrants.

Do come snd join our excellent meeting. All welcome for a great day of science of proteins in action. And all for £25!! We would love to see you and our conference venue is great too!

Poster of meeting for 28 March 2025

Are you interested in proteins as exciting, dynamic interacting species. Come and join us- March 28th 2025.

Places are limited so please register ASAP at sheena-radford-lab.uk/fida.php. (£25.00 registration only, including excellent catering).

Super day today of friendly top class science at Exeter- thank you to all involved and to the VC for a splendid introduction.

Brilliant talk (introduced by our VC Lisa Roberts) on the formation of a temporal series of amyloid aggregates in driving human diseases by Sheena Radford at our distinguished lecturer series. @lsiexeter.bsky.social a
LSI / Biosciences @ Exeter.

Job Opportunity at University of Leeds: Research Fellow in Structural Biology Are you an ambitious researcher looking for your next challenge? Do you have an established background in structural studies of amyloid aggregates and want to help to develop new routes to combat amyl...

Great opportunity to join the Radford / Ranson labs and the world-renowned Astbury Centre as a Research Fellow in Structural Biology! Please see jobs.leeds.ac.uk/vacancy.aspx...

Poster of meeting for 28 March 2025

Are you interested in proteins as exciting, dynamic interacting species. Come and join us- March 28th 2025.

Places are limited so please register ASAP at sheena-radford-lab.uk/fida.php. (£25.00 registration only, including excellent catering).

Rationalizing mAb Candidate Screening Using a Single Holistic Developability Parameter A framework for the rational selection of a minimal suite of nondegenerate developability assays (DAs) that maximize insight into candidate developability or storage stability is lacking. To address this, we subjected nine formulation:mAbs to 12 mechanistically distinct DAs together with measurement of their accelerated and long-term storage stability. We show that it is possible to identify a reduced set of key variables from this suite of DAs by using orthogonal statistical methods. We exemplify our approach by predicting the rank formulation:mAb degradation rate at 25 °C (determined over 6 months) using just five DAs that can be measured in less than 1 day, spanning a range of physicochemical features. Implementing such approaches focuses on resources, thus increasing sustainability and decreasing development costs.

And the Molecular Pharmaceutics paper:
pubs.acs.org/doi/10.1021/...

Biophysical analysis of proteins in the early drug development pipelin | BTT Therapeutic proteins, particularly monoclonal antibodies, have emerged as powerful medicines over the past forty years due to their potency. Read more.

Congratulations to Leon Willis and co-authors for getting two publications accepted recently: a review on antibody developability in Biologics and a paper where we develop an analytical workflow to assess this in Molecular Pharmaceutics. Links to both below!
www.dovepress.com/biophysical-...