Ieva Drulyte

🚨 New blog: Automated cryo-EM analysis of the gastric proton pump

We reprocessed EMPIAR-11057 using a fully automated end-to-end workflow:
⚡ 2.6 Å in 43.5 hr (vs 2.8 Å published)
⚡ 2.4 Å after CTF refinement
⚡ Improved ligand density

🔗 Full article: docs.cryocloud.io/blog/

#cryoEM #CryoCloud

Not every day your work helps protect baby elephants 🐘💙

Super proud to have contributed to the cryo-EM part of this project - it also happens to be my 20th publication, which makes it feel extra special ☺️

Honoured to be selected as one of this year’s EMBO Young Investigators!

Grateful to my team and colleagues for all their support, and excited about the opportunities this will create for my group and for our work in structural virology.

www.embo.org/press-releas...

WeINSPIREBio’s mission is to empower, connect, and elevate women scientists working in structural biology and structure-based drug design.

If you’d like to follow updates from the community, here’s the link you the WeINSPIREBio group on LinkedIn: www.linkedin.com/groups/13099...

🌟 WeINSPIREBio is hosting its first event!

On Dec 4, we’re running a webinar:

Structures of Success: Career Journeys of Women in Structural Biology

If you work in SB, drug discovery, or just like hearing career stories from brilliant people, join us! And please share 🙏🏼

lnkd.in/ewjD5WPt

#cryoEM

Heading to #PSDI2025 in Basel! Excited for good science, great discussions and familiar faces. Come say hi or book a chat with us!

Thrilled, and admittedly relieved, to see this major investment in Dutch #cryoEM become reality! 🇳🇱🔬

Honoured to be a co-applicant on this national initiative and very excited for the opportunity to advance our work in cellular structural virology! 🦠

www.uu.nl/en/news/33-m...

Celebrate #NationalCatDay with the cats of RCSB PDB!

😬

Yikes! In this paper the AlphaFold model of human plasminogen (PDB-8uq6) has been refined into noise (EMD-42462) using Phenix & ISOLDE. For some reason the authors even interpret side-chain interactions:
#cryoEM

✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv

Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo

🔗 www.biorxiv.org/content/10.1...

Germinal center–mediated broadening of B cell responses to SARS-CoV-2 booster immunization SARS-CoV-2 variant booster vaccination broadens human B cell responses through the germinal center reaction.

Read the latest from us here:

www.science.org/doi/10.1126/...

#antibody #cryoEM

Initial attempt at replicating in relion (parameters in next post). This is for Aca2-RNA, using a 100k subset of the 2D-classified particles (no prior 3D cleanup).

A 1-class ab initio in relion, then local refinement in relion (1.8deg searches+blush) gives a nominally 3.3Å map; 3.5 Å w/out blush.

📣 Live from the Dutch Structural Biology Meeting in Delft! Robert (@riborob.bsky.social) is presenting our CryoCloud poster on accelerating #cryoEM workflows. We’re also sponsoring the Poster Prize 🧦🎉 Big thanks to Arjen Jakobi (@cryotud.bsky.social) for organising. Excited for today’s discussions!

September Map of the Month: Small Data, Big Insights ⚡️

From just 233 movies, CryoCloud delivered a 3.2 Å SARS-CoV-2 spike map in 3.5 h.

Fast and efficient #cryoEM, exactly what vaccine design teams need when screening dozens of antigen variants per day.

📬 DM or hi@cryocloud.io for more info!

Coming up next week: the third Dutch Structural Biology meeting! Now in Delft, on Sept 26. See program here: nvbmb.kncv.nl/biology-2025 with #teamtomo #cryoEM #nmrchat simulations and more (looks like)

Organized by the #NVBMB (associated with KNCV)

So happy to have you on board, @jbrwhite.bsky.social!

A few snaps from @cryocloudio.bsky.social retreat in the Belgian Ardennes 📸🌿

We mixed focused work sessions with walks and shared meals, late-night Jenga battles, and plenty of time socialising. We even spotted deer in the garden 🦌

And yes, we also launched our own #cryoEM socks!

The CryoCloud team just returned from a retreat in the Belgian Ardennes 🌿 For the first time, all 11 of us met in person, with colleagues from 6 countries. We had work sessions, walks, meals, spotted deer 🦌 … and launched our first edition of CryoCloud #cryoEM socks! 🚀

August Map of the Month: Epitope Mapping edition!

We reprocessed EMD-19016 and got a 2.7 Å map of PDCoV RBD in complex with two neutralising Fabs ✨

📊 4,736 movies
⚡️ 35.5 h cloud compute
🔬 2.7 Å map from 120k particles (0.3 Å better than published)

📬 DM or hi@cryocloud.io for more info!

#CryoEM

Best thing is to come back from ⛰️vacation☀️ to find a manuscript has metamorphosed into a published paper, so..

🚀 Check out our paper on hormone import⏬ into plant cells in @natplants.nature.com 🌱👇

www.nature.com/articles/s41...

Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps Lövestam and Scheres present a cryo-EM structure of vault particles, found during tau filament extraction from progressive supranuclear palsy (PSP) brain tissue. They reveal a symmetry shift at the va...

Sofia Lövestam's vault structure is now online in Structure @cellpress.bsky.social. 🥳

How beautifully explorative is #cryoEM! We never planned to do this. The vaults just came down with #amyloids from human brain tissue, and Sofia decided to have a closer look.

www.cell.com/structure/fu...

Job Opportunity at University of Leeds: Correlative Imaging Scientist Are you a creative postdoctoral-scientist keen to explore novel in-situ biology with cryogenic electron tomography? Do you have a background in imaging technologies and/or image processing?We are look...

🚨 We’re hiring! 🚨

We’re looking for a Correlative Imaging Scientist to join our EM team at ABSL!

You will be developing and supporting cryo-EM & cryo-CLEM workflows across multiple imaging platforms (FIB-SEM, cryo-confocal, cryo-EM).

Any questions? Get in touch!

jobs.leeds.ac.uk/Vacancy.aspx...

Cryo-EM Structures Uncover Diverse L and P Protein Interactions in Nipah Virus Polymerase Among Paramyxoviruses In a groundbreaking advance that significantly deepens our understanding of viral replication machinery, researchers have unveiled high-resolution cryo-electron microscopy (cryo-EM) structures of the Nipah virus (NiV) polymerase complex. This detailed structural insight opens new avenues for the design of antiviral agents targeting a highly pathogenic zoonotic virus responsible for severe and often fatal human infections. Nipah virus, an emergent member of the Paramyxoviridae family, poses a persistent public health threat due to its zoonotic nature and the absence of approved therapeutics.

Cryo-EM Structures Uncover Diverse L and P Protein Interactions in Nipah Virus Polymerase Among Paramyxoviruses

In a groundbreaking advance that significantly deepens our understanding of viral replication machinery, researchers have unveiled high-resolution cryo-electron microscopy (cryo-EM)…

High resolution CryoEM maps of RNA assemblies

Not my area at all, but how cool are these cryoEM structures of purely RNA-based assemblies!
www.nature.com/articles/s41...

Nice cover showing cryo-EM derived structures of p53 binding to nucleosomes. Great work from the Thoma lab:
www.cell.com/molecular-ce...

🔐 Cloud Security Part 2

In this second post of our security FAQ series, we are digging into what happens behind the scenes to keep your data safe every single day.

➡️ Click through to learn more about how we manage access, monitor activity, and keep your data safe.

#CloudSecurity #CryoEM

New preprint: In situ Architecture of #Plasmodesmata.

Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.

🔗 doi.org/10.1101/2025...

🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

Cryo-EM structure of native honey bee vitellogenin | Nature Communications Vitellogenin (Vg) is the main yolk precursor lipoprotein in almost all egg-laying animals. In addition, along its evolutionary history, Vg has developed a range of new functions in different taxa. In the honey bee, Vg has functions related to immunity, antioxidant protection, social behavior and longevity. However, the molecular mechanisms underlying Vg functionalities are still poorly understood. Here, we report the cryo-EM structure of full-length honey bee Vg, one-step purified directly from hemolymph. The structure provides structural insights into the overall domain architecture, including the lipid binding cavity and the previously uncharacterized von Willebrand factor type D domain. A domain of unknown function has been identified as a C-terminal cystine knot domain based on structural homology. Information about post-translational modifications, cleavage products, metal and lipid binding allow an improved understanding of the mechanisms underlying the range of Vg functionalitie

Discover the cryo-EM structure of honey bee vitellogenin, revealing insights into its roles in immunity, antioxidant defense, behavior, & longevity. PMID:40593495, Nat Commun 2025, @NatureComms https://doi.org/10.1038/s41467-025-58575-y #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪

🔐 Cloud Security Part 1

We often get asked about cloud security – and rightly so.

In this three-part series, we’re addressing the most common questions we hear from customers.

➡️ Click through to find out how we protect your data with end-to-end encryption and built-in redundancy.

#Cloud #CryoEM