Jordan Crameri

Mitochondrial presequences harbor variable strengths to maintain organellar function. New study from Youmian Yan, Natalie Niemi (@nieminm.bsky.social) et al. @washumedicine.bsky.social: rupress.org/jcb/article/...

@ianofbristol.bsky.social
#Biochemistry #CellMetabolism #Organelles #Mitochondria

Proteome-wide in silico screening for human protein-protein interactions Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...

Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp

From Biogenesis to Breakdown: How Protein Biogenesis and Quality Control Failures Drive Mitochondrial Disease Mitochondria rely on the coordinated function of over 1000 proteins, most of which are nuclear-encoded, synthesized in the cytosol, and imported into distinct mitochondrial sub-compartments. Thirte...

From Biogenesis to Breakdown: How Protein Biogenesis and Quality Control Failures Drive Mitochondrial Disease

Online now - the Review "Interconnectivity of mitochondrial protein biogenesis and quality control" from @mitolab.bsky.social et al.

#ProteinImport #Proteaes #Proteolysis #Proteostasis

Read it here: authors.elsevier.com/sd/article/S...

Complex II assembly drives metabolic adaptation to OXPHOS dysfunction Succinate dehydrogenase assembly dynamics maintains metabolic homeostasis during mitochondrial oxidative stress.

We noticed CII assembly factor SDHAF2 accumulates in some OXPHOS deficient cell lines but not others. New Dr Kugan cracked the case: CII assembly controls a balance btwn TCA cycle direction and protection from Q-pool stress to support ROS-meditated metabolic reprogramming in OXPHOS adapted cells.

Top: HeLa cells expressing FLAGLpPIP, or FLAGRVxFdead were stained with 100 nM MitoTracker Deep Red FM (mitochondria; MTDR) and, following fixation, processed for immunofluorescence using antibodies against FLAG (green) and PPP1CB (magenta). Nuclei were stained with Hoechst 33258 (blue). Scale bar represents 10 μm. Fluorescence signal profiles of FLAG and PPP1CB were measured across the indicated line of interest. Bottom: AlphaFold3 structure of LpPIP-PPP1CB complex labeled with the active site (purple) and three substrate binding grooves (yellow) of PPP1CB.

#Legionella pumps 100s of effector proteins into host cells, including to #mitochondria. @drdstojanovski.bsky.social &co show that effector LpPIP is a tail-anchored protein of the MOM that recruits protein phosphatase 1 to modulate the host mitochondrial P-proteome @plosbiology.org 🧪 plos.io/4f2T5kE

Silencing mitochondrial gene expression in living cells Mitochondria fulfill central functions in metabolism and energy supply. They express their own genome, which encodes key subunits of the oxidative phosphorylation system. However, central mechanisms u...

Researchers in Science have developed a tool to silence specific mitochondria-encoded mRNAs in living cells, which could help scientists understand the origins of certain mitochondria-related diseases. scim.ag/3F6Wm5d

Cutting the diagnosis journey for children born with rare genetic diseases Families can wait years for a diagnosis of a rare genetic disorder, but a new test can provide answers in days for a better understanding of the condition and potentially earlier treatment, finds new...

We're super proud to see our study showing utility of proteomics in ultra-rapid variant prioritisation for suspected mito and other rare diseases out in Genome Medicine (rdcu.be/endwE). Too many amazing collabs to thank, so here are the big ones @daniellahock.bsky.social @thorburnmito.bsky.social!

Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...

@wehi-research.bsky.social @komanderlab.bsky.social

Ya-Ting Chang, @nanigrotjahn.bsky.social et al. @scripps.edu present a membrane-guided approach for identifying a subset of cytoplasmic #ribosomes oriented for protein import on the surface of #mitochondria in budding yeast using #cryoET: rupress.org/jcb/article/...

A subcellular map of translational machinery composition and regulation at the single-molecule level Millions of ribosomes are packed within mammalian cells, yet we lack tools to visualize them in toto and characterize their subcellular composition. In this study, we present ribosome expansion micros...

Two complementary methods ( #singlemoleculeimaging of ribosomal populations, specialized ribosomes and associated mRNAs & affinity purification of subcellular ribosome populations) used to characterize ribosomes in subcellular space ➡️ www.science.org/doi/10.1126/...

Mitochondria serve as a Holdout Compartment for Aggregation-Prone Proteins hindering Efficient Ubiquitin-Dependent Degradation The accumulation of protein aggregates has been causatively linked to the pathogenesis of neurodegenerative diseases. In this study, we have conducted a genome-wide CRISPR-Cas9 screen to identify cell...

Mitochondria a safe haven for aggregation-prone proteins? #neurodegeneration. #Parkinson. Find our latest manuscript in @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...

We are hiring! The Formosa Group is hiring a Research Officer to join our program on #Mitochondria, #Metabolism & #MitochondrialDisease at the Monash BDI!

💡If you're passionate about mitochondrial research or know someone who is, please share! Apply now: careers.pageuppeople.com/513/cw/en/jo...

Mouse brain organoids model in vivo neurodevelopment and function and capture differences to human In the last decade since their emergence, brain organoids have offered an increasingly popular and powerful model for the study of early development and disease in humans. These 3D stem cell-derived m...

Nice to end the year with a paper! Very excited to share my first authorship in the @lancasterlab.bsky.social lab - making mouse organoids 🧠

Check it out: doi.org/10.1101/2024...

Reactive oxygen species control protein degradation at the mitochondrial import gate This work identifies reactive oxygen species released by the electron transport chain as sentinel molecules that regulate localized protein degradation at mitochondrial TOM complexes, a mechanism that controls mitochondrial protein import and adjusts the abundance of the electron transport chain to cellular needs.

Excited to reveal specific reactive oxygen species as sentinel molecules that control localized protein degradation at #mitochondria to adjust electron transport chain abundance, with many implications for the role of ROS in development and disease.

www.cell.com/molecular-ce...

What an amazing time AussieMit 2024 was! Was honoured to get the chance to present our recently published work on TIMM50 associated mitochondrial disease. Looking forward to the next event with everyone in 2026!
#Mitochondria #AussieMit #MitochondrialDisease #Mito

Illuminate Tomorrow – This World Mitochondrial Disease week we are illuminating tomorrow’s leaders in Mito research Illuminate Tomorrow – This World Mitochondrial Disease week we are illuminating tomorrow’s leaders in Mito research

Was fortunate to speak with the MDHS team at @Unimelb recently about mitochondrial disease and the importance of basic research in developing novel therapeutics and personalized medicines!
Read here: biomedicalsciences.unimelb.edu.au/news-and-eve...
#Mitochondria #Unimelb #BasicResearch

Entire suite of Scientific colour maps (perceptually uniform and color-blind friendly) by Fabio Crameri, with hand-pickable colours of discrete and categorical colour palettes.

Scientific colour maps 8.0

· Perceptually uniform
· Perceptually ordered
· Colour-blind friendly
· Readable as B&W print
· Widely compatible
· Versioned & citable

fabiocrameri.ch/colourmaps

#useBatlow #Dataviz #SciArt #EduSky #OpenSource
1/n

Figure 4. Position and type of fluorescent tag affect protein expression and P-body numbers in vivo.

The dark side of fluorescent protein tagging: the impact of protein tags on biomolecular condensation
www.biorxiv.org/content/10.1...

Yes! I'll be presenting during the Young Investigator session

Cannot wait to hear about all the fantastic work happening in the Aussie mito community this year at AussieMit 2024!