Epigenetics Podcast

Chromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC (Steven Henikoff) | Epigenetics Podcast In this episode of the Epigenetics Podcast, we caught up with Steven Henikoff from the Fred Hutchinson Cancer Research Center in Seattle to talk about his work on Chromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC. In the last few years Steven Henikoff has been developing methods which profile the chromatin landscape by using enzyme tethering. The quest first started with ChEC-Seq, which improved on Uli Laemmli's method of Chromatin endogenous cleavage (ChEC) but used sequencing as a read-out rather than southern blotting. Next, Cleavage Under Targets & Release Using Nuclease (CUT&RUN) was developed by making a fusion protein of Protein A and micrococcal nuclease (MNase), making it possible to achieve antibody-targeted cleavage of chromatin fragments. And finally, Cleavage Under Targets & Tagmenation (CUT&Tag) was developed by using Transposase Tn5 instead of MNase, which adds sequencing adapters and fragments chromatin at the same time, streamlining the protocol even further. In this episode we discuss how working on centromeres set the stage for Steven Henikoff’s subsequent work, how he developed CUT&RUN and CUT&Tag, what the advantages and disadvantages of those methods are and how he developed all those experiments at home in his garage.   References Takehito Furuyama, Steven Henikoff (2009) Centromeric nucleosomes induce positive DNA supercoils (Cell) DOI: 10.1016/j.cell.2009.04.049 Kristina Krassovsky, Jorja G. Henikoff, Steven Henikoff (2012) Tripartite organization of centromeric chromatin in budding yeast (Proceedings of the National Academy of Sciences of the United States of America) DOI: 10.1073/pnas.1118898109 Jorja G. Henikoff, Jitendra Thakur, … Steven Henikoff (2015) A unique chromatin complex occupies young α-satellite arrays of human centromeres (Science Advances) DOI: 10.1126/sciadv.1400234 Epigenomics Methods: ChEC-Seq, CUT&RUN, AutoCUT&RUN, Improved CUT&RUN, CUT&Tag, Automated CUT&Tag, CUTAC, scCUT&Tag.   Related Episodes The Role of Non-Histone Proteins in Chromosome Structure and Function During Mitosis (Bill Earnshaw) Hi-C and Three-Dimensional Genome Sequencing (Erez Lieberman Aiden) In Vivo Nucleosome Structure and Dynamics (Srinivas Ramachandran)   Contact Active Motif on Twitter Epigenetics Podcast on Twitter Active Motif on LinkedIn Active Motif on Facebook Email: podcast@activemotif.com

CUT&Tag and CUT&RUN: Redefining the Standards for Protein–DNA Mapping in Epigenetics: https://epigenetics-podcast.micro.blog/2025/07/30/cuttag-and-cutrun-redefining-the.html

In the most recent episode we talked with Dr. Joseph Ecker from the Salk Institute about his work on high-resolution genome-wide mapping technologies in Arabidopsis and the human brain. #podcast #chromatin #epigenetics

Listen here: activemotif.com/podcasts-jos...

In this episode we talked with Dr. Joseph Ecker from the Salk Institute about his work on high-resolution genome-wide mapping technologies in Arabidopsis and the human brain. #podcast #chromatin #epigenetics

Listen here: activemotif.com/podcasts-jos...

In the most recent episode we talked with @teichlab.bsky.social from @cam.ac.uk about the Human Cell Atlas. #podcast #epigenetics #chromatin #singlecell

Listen here: www.activemotif.com/podcasts-sar...

In this episode we talked with @teichlab.bsky.social from @cam.ac.uk about the Human Cell Atlas. #podcast #epigenetics #chromatin #singlecell

Listen here: www.activemotif.com/podcasts-sar...

In the most recent episode, Professor Asim Surani, shares how his extensive research has significantly advanced the understanding of how the mammalian germline is specified. #chromatin #podcast #epigenetics

Listen here: activemotif.com/podcasts-azi...

In this episode, Professor Asim Surani, shares how his extensive research has significantly advanced the understanding of how the mammalian germline is specified. #chromatin #podcast #epigenetics

Listen here: activemotif.com/podcasts-azi...

2/3 on the Epigenetics Podcast :)

East coast chromatin mafia
@kjarmache.bsky.social

🔎 Despite failed experiments as a student, alumnus Professor Azim Surani has spent his entire career trying to understand early mammalian development.

🔬 This year marks the 40th anniversary of his discovery of genomic imprinting — the process that tags specific genes, turning

In the most recent episode of the Epigenetics Podcast, Petra Hajkova from the @mrc-lms.bsky.social‬ discusses her research on mammalian development, focusing on DNA methylation, histone modifications and TET enzymes. #podcast #epigenetics

🎧 Listen here: activemotif.com/podcasts-pet...

In this episode of the Epigenetics Podcast, Petra Hajkova from the @mrc-lms.bsky.social‬ discusses her research on mammalian development, focusing on DNA methylation, histone modifications and TET enzymes. #podcast #epigenetics

🎧 Listen here: activemotif.com/podcasts-pet...

🎙️ Catch Ani Deshpande, PhD, in episode 151 of the @epigeneticspod.bsky.social‬‬ where he discusses his work on epigenetic regulation in #AML, and the use of #CRISPR and high throughput screens for drug discovery.

▶️ YouTube: vist.ly/3n5jemi
ᯤ Spotify: vist.ly/3n5jemj

BEFORE THE BATHROOM SCALE WAS INVENTED, THE ONLY WAY TO WEIGH PEOPLE WAS MASS SPECTROMETRY.

Love today’s #xkcd comic on
#MassSpec
🤣

In the most recent episode, I talked with @anideshpande.bsky.social from @sbpdiscovery.bsky.social about his work on epigenetic regulation and developing small molecules through high throughput screens for AML. #epigenetics #podcast #chromatin #cancer

Listen here: activemotif.com/podcasts-ani...

In this episode, we talked with @anideshpande.bsky.social from @sbpdiscovery.bsky.social about his work on epigenetic regulation and developing small molecules through high throughput screens for AML. #epigenetics #podcast #chromatin #cancer #leukemia

Listen here: activemotif.com/podcasts-ani...

Last week we were hyped to celebrate our 150th Episode. We had the pleasure to talk to Raffaele Teperino about the Paternal Influence in Epigenetic Inheritance. #epigenetics #podcast

Listen here: www.activemotif.com/podcasts-raf...

It's good to be back!

#EMBLChromatin

Can a father's health before conception shape future generations?
In our most recent episode, Dr. Raffaele Teperino from @epihmgu.bsky.social unpacks the science behind paternal effects, epigenetics, and metabolic disruption.

Listen now: activemotif.com/podcasts-raf...
#Epigenetics #SciencePodcast

Can a father's health before conception shape future generations?

In our latest episode, Dr. Raffaele Teperino from @epihmgu.bsky.social unpacks the science behind paternal effects, epigenetics, and metabolic disruption.

Listen now: activemotif.com/podcasts-raf...
#Epigenetics #SciencePodcast

In the most recent episode we talked with Dr. Frank Johannes from @tum.de in Freising about his work on evolutionary clocks and epigenetic inheritance in plants. #podcast #epigenetics

Listen here: activemotif.com/podcasts-fra...

In this episode we talked with Dr. Frank Johannes from @tum.de in Freising about his work on evolutionary clocks and epigenetic inheritance in plants. #podcast #epigenetics

Listen here: activemotif.com/podcasts-fra...

Stylized illustration of a microphone in the center, surrounded by symmetrical audio waveforms on a blue gradient background, representing audio recording or a podcast

In the new episode of the We are TUM #podcast, we visit our #Heilbronn #campus and hear from those who are helping to shape the location - from the #student perspective to strategic visions for the coming years. Listen now (in German): go.tum.de/244301 🎙️

📷ediundsepp

Excited to share our latest work on how cells fold their genomes, and build mitotic chromosomes! Out in Science today!

Lots of interesting results and proposals for those interested in loop extrusion, and chromosomes!

With @golobor.bsky.social, Leonid Mirny, Bill Earnshaw labs.

See thread below:

In the most recent episode we talked with @boyanbonev.bsky.social from @bonevlab.bsky.social at @epihmgu.bsky.social about his work on neuroepigenetics, focusing on gene regulation, chromatin architecture, and primate epigenome evolution. #epigenetics

Listen here: activemotif.com/podcasts-boy...

In this episode we talked with @boyanbonev.bsky.social from @bonevlab.bsky.social at @epihmgu.bsky.social about his work on neuroepigenetics, focusing on gene regulation, chromatin architecture, and primate epigenome evolution. #podcast #epigenetics

Listen here: activemotif.com/podcasts-boy...

Miloshev et al., 2025, Linker Histones Maintain Genome Stability and Drive the Process of Cellular Ageing [review] www.imrpress.com/journal/FBL/...

Interesting figure: we did observe NRL increase with ageing (onlinelibrary.wiley.com/doi/10.1111/...). Not sure about about loop sizes, to be checked

Check this exciting new program out and apply! My lab is also recruiting scientists in this call for #stemcell and #mechanobiology related projects

In the most recent episode we talked with @evahoermanseder.bsky.social from @www.helmholtz-munich.de‬ about her work on epigenetic mechanisms in cellular memory and gene regulation. #podcast #epigenetics

Listen here: activemotif.com/podcasts-eva...

#EpigeneticsPodcast with #HelmholtzMunich expert Dr. Eva Hörmanseder, @epihmgu.bsky.social!

🎧 Listen and learn about epigenetic memories & more:
👉 t1p.de/30q54

👉Check out her latest findings on this topic:
t1p.de/43yrw

@epigeneticspod.bsky.social
#Epigenetics #Podcast #Reprogramming #CellFate