Raphael Ferreira

Our work on nucleic acid sensing in live human cells is out 🧬⚫️🟢! preprint: www.biorxiv.org/content/10.1...

16.03.2025 10:10 — 👍 1    🔁 1    💬 0    📌 0

We're hiring to expand on the work to understand the human genome by engineering it!
lnkd.in/da-gitNc

10.02.2025 08:53 — 👍 12    🔁 8    💬 0    📌 0

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬

The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy

www.biorxiv.org/content/10.1...

10.02.2025 08:21 — 👍 316    🔁 101    💬 15    📌 13

Sequence-dependent activity and compartmentalization of foreign DNA in a eukaryotic nucleus In eukaryotes, DNA-associated protein complexes coevolve with genomic sequences to orchestrate chromatin folding. We investigate the relationship between DNA sequence and the spontaneous loading and a...

Our latest work: how can compartmentalization emerge in a eukaryotic genome lacking canonical heterochromatin?
By investigating bacterial genomes put in yeast, we show that the presence or absence of transcription is sufficient!
#chromatin #3Dgenome #generegulation
www.science.org/doi/10.1126/...
👇

07.02.2025 10:22 — 👍 185    🔁 76    💬 3    📌 10

🧬@science.org Randomizing the human genome by engineering recombination between repeat elements bit.ly/4jyT4Hf @jonaskoeppel.bsky.social @f-raphael.bsky.social @geochurch.bsky.social @proftomellis.bsky.social @leopoldparts.bsky.social +al. @sangerinstitute.bsky.social @harvardmed.bsky.social #synbio

01.02.2025 18:48 — 👍 10    🔁 5    💬 0    📌 0

Genome recombination on demand Large genome rearrangements in mammalian cells can be generated at scale

@science.org Genome recombination on demand | Science www.science.org/doi/10.1126/... a Perspective by @seczmarta.bsky.social Lars Steinmetz @stanford.edu on two studies bit.ly/4hzFRMg + bit.ly/4jyT4Hf that generate large genome rearrangements in mammalian cells @ unprecedented scale #synbio #genome

01.02.2025 18:58 — 👍 47    🔁 20    💬 1    📌 0

Randomizing the human genome by engineering recombination between repeat elements We lack tools to edit DNA sequences at scales necessary to study 99% of the human genome that is noncoding. To address this gap, we applied CRISPR prime editing to insert recombination handles into re...

We're delighted to share our work on scrambling the human genome using prime editing, repetitive elements, and recombinases in @science.org , led by @jonaskoeppel.bsky.social , @f-raphael.bsky.social , with @proftomellis.bsky.social and George Church.
www.science.org/doi/10.1126/...

31.01.2025 13:48 — 👍 139    🔁 61    💬 2    📌 5

Enhancer scrambling strategy

We are happy to share our enhancer scramble story, a strategy to create hundreds of stochastic deletions, inversions, and duplications within mammalian gene regulatory regions and associate these new architectures with gene expression levels 🧵
www.biorxiv.org/content/10.1...

15.01.2025 20:32 — 👍 182    🔁 77    💬 3    📌 2