Daniel Krüger

Thanks a lot, Jared!!

Thanks a lot!

Beautiful high-res work from the Delacour lab: a basal supracellular ‘actin-star’ network in intestinal villi. With imaging, ablation and myosin perturbations, they support a tissue-level actomyosin tension scaffold— another great companion to our extrusion findings.
www.nature.com/articles/s41...

Great to see our conclusions align with the beautiful work from @marija-matejcic.bsky.social in the Trepat lab 3D force mapping, Ca²⁺-triggered myosin-IIA meshwork dissolution, and neighbour lamellipodia generating traction. Great synergy, highlighting the role of tension in extrusion regulation.

I feel like this is the most rewarding comment I ever got.

Thanks for highlighting this! My background in fly morphogenesis shaped how I think about tissue forces, their interplay with signaling, and experimental design. An experience I’d wholeheartedly recommend — Drosophila remains a rich source of inspiration, continuing to reveal fundamental concepts.

This is very complementary with the recent preprint of @xaviertrepat.bsky.social and Maria Matejcic using 2D organoids and providing 3D mechanical characterisation of the process

Important story dissecting the mechanism of gut cell extrusion using organoids. Local heterogeneity of tension promotes live extrusion and basal relaxation is sufficient to kick the cell out. Nice combination of optogenetics and live imaging to demonstrate this
www.science.org/doi/abs/10.1...

👉 Check out our key findings in this detailed thread by Hans Clevers.

Grateful for my time @thecleverslab.bsky.social with @hansclevers.bsky.social to all collaborators @hubrechtinstitute.bsky.social , @amolf-nl.bsky.social & @IHB Basel as well as to MSCA @ec.europa.eu programme for supporting this journey. Couldn’t have done it without the amazing support!

The gut constantly renews itself. Cells keep the tissue under tension, engaging in a tug-of-war. Weaker cells that can’t counter their neighbours’ pull extrude. This maintains mechanical homeostasis, ensuring stability and protecting the intestinal barrier.

Epithelial tension controls intestinal cell extrusion Cell extrusion is essential for homeostatic self-renewal of the intestinal epithelium. Extrusion is thought to be triggered by crowding-induced compression of cells at the intestinal villus tip. In th...

Out in @science.org! We found that the intestine doesn’t just push out cells when crowded. Instead, cells are in a mechanical tug-of-war, and weaker ones are extruded. A new view of how our gut keeps its balance and stays healthy.
www.science.org/doi/10.1126/...

📣 It is my pleasure to announce our recent review with Margherita on the current approaches, challenges, and perspectives in studying human maternal-fetal interface using in vitro models www.cell.com/cell-stem-ce... 🚀✨️

Jeey! @nfdi4bioimage.bsky.social published their #RDM-related postcards FAIRly (made by Henning Falk, licensed CC-BY 4.0) 🔬💾😍
github.com/NFDI4BIOIMAG...

First time that my cartoons are printed on postcards! Have a look 👇

Long-term in vitro expansion of a human fetal pancreas stem cell that generates all three pancreatic cell lineages Identification of an LGR5 as a marker for a tripotent stem/progenitor cell of the human fetal pancreas. Organoids derived from single LGR5+ cells are capable of long-term expansion in vitro and genera...

Exciting study by Amanda Andersson Rolf et al: In vitro expansion of a human fetal #pancreas #stemcell that generates all three pancreatic cell lineages”. www.cell.com/cell/fulltex...
New #organoid protocol allows the seemingly indefinite expansion of human fetal pancreatic Lgr5+ stem cell 1/

Europe’s east-west divide is widening Relations between Germany and Poland define the continent. But, ahead of the latter’s election this weekend, they are in crisis and getting worse.

"The state of German-Polish relations is a European tragedy in the same way that the Warschauer Kniefall was a moment of European reconciliation."
Brilliant analysis by @JeremyCliffe and also an important read before the Polish elections this weekend.

There is so much at stake.

Warum Investoren immer mehr Ackerland kaufen Überregionale Investoren kaufen immer mehr Ackerland in Sachsen-Anhalt auf, die Preise sind stark gestiegen. Seit der Energiewende gibt es noch mehr Konkurrenz.

Anhören!
Wie sich große branchenfremde Investoren (ALDI, Immobilien etc) riesengroße Ackerflächen 🚜 in Ostdeutschland kaufen.
Am Staat vorbei, ohne Steuern zu zahlen, mit dem Segen des Bauernverbandes und zum Schaden der kleinen Landwirtschaftsbetriebe.
👇

Jugendarbeitslosigkeit in der EU, August 2023:

🇪🇸26.8%
🇪🇪23.5%
🇬🇷22.5%
🇷🇴22.3%
🇮🇹22.0%
🇸🇪21.1%
🇵🇹20.3%
🇭🇷20.0%
🇸🇰19.8%
🇱🇺19.2%
🇨🇾16.3%
🇫🇮16.1%
🇫🇷16.0%
🇧🇪14.2%
🇪🇺14.0%
🇭🇺12.8%
🇧🇬11.8%
🇩🇰11.6%
🇦🇹11.4%
🇮🇪11.2%
🇵🇱11.1%
🇸🇮10.4%
🇱🇻9.0%
🇲🇹9.0%
🇳🇱8.7%
🇱🇹8.6%
🇨🇿8.5%
🇩🇪5.7%

via: ec.europa.eu/eurostat/doc...

Möglicherweise ist es sogar hilfreich das zu tun. Wenn diese Plattform ehm. Twitter ersetzt, Informationsaustausch und demokratischen Diskurs ermöglicht, bedeutet es auch dass die Zerstörung einer Institution allein nicht genügt. Es zeut vielmehr von demokratischer Resilienz.

BlueSkye, let's go!