Ca2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
Lysosomes are essential in maintaining cellular health. Endocytic lysosome reformation (ELR) regenerates functional lysosomes following degradation of endocytic cargo, yet the mechanisms driving this process remain largely unknown. Here, we define the molecular machinery underlying ELR. We find that unlike autophagic lysosome reformation (ALR), ELR proceeds independently of mTOR and dynamin 2, but requires the mitochondrial fission GTPase DRP1. DRP1 mediates scission of endolysosomal tubules at contact sites with the endoplasmic reticulum (ER) and mitochondria. Disruption of DRP1 function or ER endolysosome contact results in elongated tubules, indicating defective lysosome reformation. Moreover, mitochondrial activity is essential for tubule initiation, and Ca2+ transfer from endolysosomes to mitochondria is crucial for ELR onset. Our findings reveal a dual role for mitochondria in ELR: first in ELR initiation and second in DRP1-dependent tubule fission at ER-mitochondria-endolysosome tripartite contact sites, uncovering the previously unappreciated role of mitochondria in endolysosome remodeling and fission. ### Competing Interest Statement The authors have declared no competing interest. Swiss National Science Foundation, 320030-231859, 310030-197779 University of Basel, https://ror.org/02s6k3f65
Check out our newest findings on how #lysosomes are generated from #endolysosomes. Great work from @samit2104.bsky.social ! @biozentrum.unibas.ch , @unibas.ch #cellbio #cell #organelles
Ca2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites www.biorxiv.org/content/10.6...
30.01.2026 17:59 — 👍 25 🔁 11 💬 1 📌 1
The small #GTPase Rab1 is a master regulator of #Golgi traffic and #autophagy. van Vliet, Munro et al. @mrclmb.bsky.social performed a proteomic screen to identify novel Rab1 effectors, including a #dynein motor adaptor and cargo receptors for autophagy. rupress.org/jcb/article/...
#Trafficking
07.01.2026 17:45 — 👍 17 🔁 12 💬 0 📌 1
MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony.
Or from a single dish of HEK cell culture in the case of two membrane proteins.
Out in Nature Methods now! lnkd.in/gpyBSceg
Wonderful collaboration with the Efremov lab.
14.11.2025 18:38 — 👍 94 🔁 44 💬 3 📌 5
Beautiful new structure of the VPS13C lipid transfer protein from the Reinisch and De Camilli labs. @pdc-lab.bsky.social
11.11.2025 22:50 — 👍 7 🔁 2 💬 0 📌 0
🚨🧠🔬 A major breakthrough in molecular neuroscience:
I am excited to share a new story from our lab, published in accelerated format today by @nature.com:
"Delta-type glutamate receptors are ligand-gated ion channels"
Read more here (free article link): rdcu.be/eGIKz
16.09.2025 16:47 — 👍 96 🔁 34 💬 10 📌 2
Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!
Make my day and check it out!
www.biorxiv.org/content/10.1...
#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬
30.07.2025 15:28 — 👍 99 🔁 31 💬 6 📌 0
Many congratulations Brett and team. Exciting findings
31.07.2025 01:36 — 👍 1 🔁 0 💬 0 📌 0
I am excited to share our latest preprint presenting the structure of human NHE6, a key endosomal Na⁺/H⁺ exchanger involved in Christianson syndrome.
We bring together #cryo-EM, NMR, SAXS, lipidomics, and functional assays for an integrative analysis of NHE6 architecture, function, and regulation.
28.07.2025 21:31 — 👍 35 🔁 11 💬 1 📌 0
Congratulations Kevin and Brett! Great work
16.04.2025 21:17 — 👍 1 🔁 0 💬 1 📌 0
Excellent work from the lab of Ben Engel.
21.03.2025 04:01 — 👍 1 🔁 0 💬 0 📌 0
Arrayed CRISPR/Cas9 Loss-Of-Function Screen in a Neuronal Model of Adaptor Protein Complex 4 Deficiency Identifies Modulators of ATG9A Trafficking https://www.biorxiv.org/content/10.1101/2025.02.22.639634v1
23.02.2025 23:31 — 👍 5 🔁 3 💬 0 📌 0
Thank you!
14.02.2025 17:23 — 👍 0 🔁 0 💬 0 📌 0
Greater than the sum of its parts: the role of VARP-SNX27 binding in endosomal “supercomplex” formation
In cells, multi-subunit coat protein complexes are responsible for coordinating the transport of transmembrane proteins and lipid cargo between membranes. At the endosome, cargo trafficking is medi…
New work from the @lpjacksonlab.bsky.social uses a combination of biochemical, biophysical and computational modeling techniques to investigate endosomal trafficking players. Congrats to 1st author @mintuju.bsky.social!
bit.ly/4hYry4o
@vubasicsciences.bsky.social @vandybiomed.bsky.social
14.02.2025 15:03 — 👍 6 🔁 3 💬 1 📌 0
Thrilled to have my first-author publication in Science Advances, and grateful to my supervisor for sharing it on Bluesky! This marks a major milestone in our journey to unravel the complexities of endosomal protein interactions!
13.02.2025 19:23 — 👍 2 🔁 1 💬 1 📌 0
VARP binds SNX27 to promote endosomal supercomplex formation on membranes
VARP binds SNX27 to promote the assembly of the SNX27/Retromer/ESCPE-1 endosomal supercomplex on membranes.
The Jackson lab is thrilled to join Bluesky and find so many friends, scientists, and colleagues. It is an uncertain and frankly scary time for U.S. science, but today we are pausing to celebrate an exciting new paper in Science Advances:
www.science.org/doi/10.1126/.... (Thread)
13.02.2025 18:10 — 👍 12 🔁 1 💬 1 📌 1
I'm into chord changes and cell biology.
Professor - University of Warwick. Director - The Company of Biologists. Views are my own and not those of any organisation I am associated with.
Mastodon: @steveroyle@biologists.social
Lab: https://roylelab.org
Laboratory of Pietro De Camilli - Department of Neuroscience at Yale University
The Graham Lab at Vanderbilt University. We study mechanism controlling protein and lipid homeostasis.
https://my.vanderbilt.edu/grahamlab/
Cell and Molecular Biologist, Cricket enthusiast
Solver of GTPases molecular puzzles | Membranes | CRISPR | STED super-res |Accidental immunologist | Academic mother of 2 cheeky monkeys | Assistant prof at FU Berlin. | Head of the Nanoscale Membrane Dynamics Lab
We are scientists interested in understanding the basics of how a cell, the building block of our body, works.
@biozentrum
@UniBasel_en
#cellbio #Biozentrum #RNA #organelles #membranes #aging #stress #proteostasis #signaling
Scientist, 2017 Chemistry Nobel Prize (math and computational methods underlying cryo-EM of biological molecules), fiction writer, photographer, gardener.
Climate change, science education, fight of fascism
franxfiction.com and joachimfranklab.org
Computational protein research group of Prof Ben Corry.
We love molecular dynamics and all kinds of membrane proteins including ion channels, transporters and immune receptors
~Posts by group members
CERM/CIRMMP is a NMR infrastructure for Life Sciences, which provides a unique environment for research in the field of Structural Biology.
https://www.cerm.unifi.it/
Multidisciplinary research at UMass Chan focused on membrane trafficking. Striving to make academic science a more diverse, equitable, welcoming and inclusive place. President of the American Society for Cell Biology (ASCB).
Opinions are mine
(she/her)
Structural biology, biophysics, biochemistry, molecular and cellular pharmacology | nuclear receptors | NMR | Vanderbilt University, Department of Biochemistry | kojetinlab.org
Vanderbilt Center for Structural Biology - Research dedicated to determining the 3D structure of biological macromolecules at or near atomic resolution.
www.vanderbilt.edu/csb
Cryo-electron tomography and subtomogram averaging. Pushing buttons, turning knobs, and furiously smashing keyboard. Assistant prof at Vanderbilt University; I ask other people to pipette things, often viruses.
PhD Candidate | Jackson Lab | Vanderbilt University
Interested in sharing my love of science with the world and making STEM education more accessible to all!
cellarchlab.com Univ. of Basel Biozentrum🇨🇭. Exploring molecular architecture inside cells with #CryoEM #TeamTomo. Plants and algae in a changing climate. ❄🔬 OF 🌿 4THE 🌍!
CryoEM, Structural Cell Biology. Group Leader@University of Melbourne. #NHMRC Emerging Leadership Fellow. Formerly at @Caltech, @MRC_LMB.
We are a cell biology lab at UT Southwestern Medical Center in Dallas, TX. We study lipid droplets and the organization of metabolism in cells. For more, please see our lab website: https://labs.utsouthwestern.edu/henne-lab
CryoEM, Bioenergetics, V-ATPases (especially in synaptic vesicles and lysosomes), OxPhos (especially in mycobacteria as a TB drug target)
Scientist, The Hospital for Sick Children
Professor, U of Toronto
👨👩👧🔬🚲
rubinsteinlab.org
COI: structura.bio
Structural virology group leader at Umeå University, Sweden. www.carlsonlab.se
membranes, autophagy, lysosomes, cryo-EM and -ET, Parkinson's
