Broder Schmidt

Direct ionic stress sensing and mitigation by the transcription factor NFAT5 Intracellular ionic strength is sensed and regulated by a prion-like domain in a transcription factor.

Ever wondered how mammalian cells sense osmotic stress? There's gotta be a signaling pathway, right?

We propose a *one-component system*: NFAT5, the transcription factor that executes the osmoprotective response, is also the stress sensor!

www.science.org/doi/full/10....

It’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.

Quick poll: what year is the below abstract from?

„Our results indicate that protein sequences contain not only a folding code, but also a previously unrecognized code governing their distribution to diverse subcellular compartments.“

Dunno what they have planned, but a devastated NIH will destroy universities and academic science (on the heels of significant COVID impacts) but will also destroy biotech and cede US leadership in this sector to China and elsewhere very very quickly.

The Dept. of Biochem. & Biophys. @ WashU is running its summer undergrad research program!

Rising seniors who have not had the opportunity to do bona fide research but are interested in this are strongly encouraged to apply

Deadline: Jan 31st
Details: biochem.wustl.edu/resources/in...

Pls share!

iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background la...

Proud to share our work spear-headed by PhD student Tommy Sroka with major contributions by #xenopus expert Kerstin Feistel.

Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.

Congrats, Alex & team! You are a force and inspiration.

True! Plus it provides an interesting new rationale to drug IDRs “across the stereochemical trees of life”

A ‘Second Tree of Life’ Could Wreak Havoc, Scientists Warn Research on so-called mirror cells, which defy fundamental properties of living organisms, should be prohibited as too dangerous, biologists said.

Maybe not the ‘chiral’ response you were expecting? www.nytimes.com/2024/12/12/s...

I recall that dissecting and drawing this parasitic worm took everything I got. Couldn’t even finish the gut epithelium…

Personally, I’m still most drawn to the protist world.

Found some old drawings I had to do for my undergrad in Molecular & Cellular Biology. @dadrummond.bsky.social may particularly appreciate this one, although I’m not nearly the artist he is.

Lol, now we know your stance on the great olive oil rivalry between Italy and Spain.

Chapter 2 Strategies and Considerations for Protein Purifications Prior to embarking upon the purification of a protein, one should begin by considering what the protein is to be used for. In particular, how much of …

Old-y but gold-y: www.sciencedirect.com/science/arti...

@boeynaemssteven.bsky.social New night science project?