Lechtenberg Lab

We are still accepting abstracts for selected short talks and posters. The abstract deadline has been extended to this Friday, 1st Aug. More information and registration here: www.lorneubiquitin.org .

📣📣 Lorne Ubiquitin – The Australian Ubiquitin Summit 📣📣 20-23 Nov 2025 in Lorne, Australia Join us this Nov for the first ubiquitin meeting in Australia. Submit your abstract by 18 July 2025 for oral and poster presentations: www.lorneubiquitin.org.
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We hope this will be useful for the field. A plasmid for expressing the constitutively active HOIL-1 variant is available via @addgene.bsky.social: www.addgene.org/229539/

Excited to share our latest work, now out in @lsajournal.org. we show that the E3 ligase HOIL-1 can ubiquitinate many different saccharides and describe a chemoenzymatic platform to generate ubiquitinated sugars as tools for studying non-proteinaceous ubiquitination.

For those interested in ubiquitinating their own sugars: our plasmid for bacterial expression of the constitutive HOIL-1 variant is now available via Addgene: www.addgene.org/229539/

The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-proteinaceous substrates in vitro https://www.biorxiv.org/content/10.1101/2024.11.29.626046v1 HOIL-1 is a RING-between-RING (RBR)-family E3 ubiquitin ligase and component of the linear ubiquitin

The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-proteinaceous substrates in vitro https://www.biorxiv.org/content/10.1101/2024.11.29.626046v1

The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro HOIL-1 is a RING-between-RING (RBR)-family E3 ubiquitin ligase and component of the linear ubiquitin chain assembly complex (LUBAC). While most E3 ubiquitin ligases conjugate ubiquitin to protein lysi...

Thanks to all the members of my lab as well as collaborators and co-authors in the Komander (WEHI), Goddard-Borger (WEHI) and Payne (Uni Sydney) labs.

www.biorxiv.org/content/10.1...

We hope that our protocol will enable us and others to generate various ubiquitinated saccharides as tools to further develop the non-proteinaceous ubiquitin field. A plasmid for bacterial expression of the HOIL-1 variant will be available at @addgene.bsky.social soon.

We utilise HOIL's broad saccharide substrate specificity to develop a simple protocol for the chemoenzymatic generation of preparative amounts of ubiquitinated saccharides. Our approach is vastly simplified by our engineered constitutively active HOIL-1 variant.

We find that HOIL-1 efficiently ubiquitinates hydroxyl groups in Ser and a wide variety of mono- and disaccharides. We show that HOIL-1's His510 residues is critical for this activity and that mutation of His510 to Ala switches HOIL-1 activity from hydroxyl groups to targeting amines.

HOIL-1 is an E3 ubiquitin ligase that can ubiquitinate non-proteinaceous substrates such as glycogen and maltose as first shown in work by @iankelsall.bsky.social. In our work, we systematically compare HOIL-1's in vitro activity against a number of amino acids (Lys, Ser, Thr) and saccharides.

The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro HOIL-1 is a RING-between-RING (RBR)-family E3 ubiquitin ligase and component of the linear ubiquitin chain assembly complex (LUBAC). While most E3 ubiquitin ligases conjugate ubiquitin to protein lysi...

Excited to share our latest preprint on @biorxivpreprint.bsky.social: "The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro".

www.biorxiv.org/content/10.1...

Check out this list of ubiquitin and Ubl profiles to follow. Repost if you want me to add you to it. Let’s create a good community here ☁️ go.bsky.app/3VPQofn